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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-1592
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dc.contributor.authorJossen, Valentin-
dc.contributor.authorSchirmer, Cedric-
dc.contributor.authorMostafa Sindi, Dolman-
dc.contributor.authorEibl-Schindler, Regine-
dc.contributor.authorKraume, Matthias-
dc.contributor.authorPörtner, Ralf-
dc.contributor.authorEibl, Dieter-
dc.date.accessioned2018-01-31T10:34:49Z-
dc.date.available2018-01-31T10:34:49Z-
dc.date.issued2016-
dc.identifier.issn1687-9678de_CH
dc.identifier.issn1687-966Xde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/2352-
dc.description.abstractThe potential of human mesenchymal stem cells (hMSCs) for allogeneic cell therapies has created a large amount of interest. However, this presupposes the availability of efficient scale-up procedures. Promising results have been reported for stirred bioreactors that operate with microcarriers. Recent publications focusing on microcarrier-based stirred bioreactors have demonstrated the successful use of Computational FluidDynamics (CFD) and suspension criteria (NS1u,NS1) for rapidly scaling up hMSC expansions from mL- to pilot scale. Nevertheless, one obstacle may be the formation of large microcarrier-cell-aggregates, which may result in mass transfer limitations and inhomogeneous distributions of stem cells in the culture broth.The dependence of microcarrier-cell-aggregate formation on impeller speed and shear stress levels was investigated for human adipose derived stromal/stem cells (hASCs) at the spinner scale by recording the Sauter mean diameter (d 32) versus time. Cultivation at the suspension criteria provided d 32 values between 0.2 and 0.7 mm, the highest cell densities (1.25 × 106 cells mL−1 hASCs), and the highest expansion factors (117.0 ± 4.7 on day 7), while maintaining the expression of specific surface markers. Furthermore, suitability of the suspension criterion NS1u was investigated for scaling up microcarrier-based processes in wave-mixed bioreactors for the first time.de_CH
dc.language.isoende_CH
dc.publisherSage-Hindawide_CH
dc.relation.ispartofStem Cells Internationalde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleTheoretical and practical issues that are relevant when scaling up hMSC microcarrier production processesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.publisher.placeNew Yorkde_CH
dc.identifier.doi10.21256/zhaw-1592-
dc.identifier.doi10.1155/2016/4760414de_CH
zhaw.funding.euNode_CH
zhaw.issue4760414de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume2016de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Jossen, V., Schirmer, C., Mostafa Sindi, D., Eibl-Schindler, R., Kraume, M., Pörtner, R., & Eibl, D. (2016). Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes. Stem Cells International, 2016(4760414). https://doi.org/10.21256/zhaw-1592
Jossen, V. et al. (2016) ‘Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes’, Stem Cells International, 2016(4760414). Available at: https://doi.org/10.21256/zhaw-1592.
V. Jossen et al., “Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes,” Stem Cells International, vol. 2016, no. 4760414, 2016, doi: 10.21256/zhaw-1592.
JOSSEN, Valentin, Cedric SCHIRMER, Dolman MOSTAFA SINDI, Regine EIBL-SCHINDLER, Matthias KRAUME, Ralf PÖRTNER und Dieter EIBL, 2016. Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes. Stem Cells International. 2016. Bd. 2016, Nr. 4760414. DOI 10.21256/zhaw-1592
Jossen, Valentin, Cedric Schirmer, Dolman Mostafa Sindi, Regine Eibl-Schindler, Matthias Kraume, Ralf Pörtner, and Dieter Eibl. 2016. “Theoretical and Practical Issues That Are Relevant When Scaling up hMSC Microcarrier Production Processes.” Stem Cells International 2016 (4760414). https://doi.org/10.21256/zhaw-1592.
Jossen, Valentin, et al. “Theoretical and Practical Issues That Are Relevant When Scaling up hMSC Microcarrier Production Processes.” Stem Cells International, vol. 2016, no. 4760414, 2016, https://doi.org/10.21256/zhaw-1592.


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