Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor

Niu, Qiuhong; Liu, Suyao; Yin, Mingshen; Lei, Shengwei; Rezzonico, Fabio; Zhang, Lin

doi:10.3390/pathogens11010082

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-23927

Full metadata record

DC Field	Value	Language
dc.contributor.author	Niu, Qiuhong	-
dc.contributor.author	Liu, Suyao	-
dc.contributor.author	Yin, Mingshen	-
dc.contributor.author	Lei, Shengwei	-
dc.contributor.author	Rezzonico, Fabio	-
dc.contributor.author	Zhang, Lin	-
dc.date.accessioned	2022-01-14T13:39:32Z	-
dc.date.available	2022-01-14T13:39:32Z	-
dc.date.issued	2022-01-10	-
dc.identifier.issn	2076-0817	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/23927	-
dc.description.abstract	Symbiotic microorganisms in the intestinal tract can influence the general fitness of their hosts and contribute to protecting them against invading pathogens. In this study, we obtained isolate Phytobacter diazotrophicus SCO41 from the gut of free-living nematode Caenorhabditis elegans that displayed strong colonization-resistance against invading biocontrol bacterium Bacillus nematocida B16. The colonization-resistance phenotype was found to be mediated by a 37-kDa extracellular protein that was identified as flagellin (FliC). With the help of genome information, the fliC gene was cloned and heterologously expressed in E. coli. It could be shown that the B. nematocida B16 grows in chains rather than in planktonic form in the presence of FliC. Scanning Electronic Microscopy results showed that protein FliC-treated B16 bacterial cells are thinner and longer than normal cells. Localization experiments confirmed that the protein FliC is localized in both the cytoplasm and the cell membrane of B16 strain, in the latter especially at the position of cell division. ZDOCK analysis showed that FliC could bind with serine/threonine protein kinase, membrane protein insertase YidC and redox membrane protein CydB. It was inferred that FliC interferes with cell division of B. nematocidal B16, therefore inhibiting its colonization of C. elegans intestines in vivo. The isolation of P. diazotrophicus as part of the gut microbiome of C. elegans not only provides interesting insights about the lifestyle of this nitrogen-fixing bacterium, but also reveals how the composition of the natural gut microbiota of nematodes can affect biological control efforts by protecting the host from its natural enemies.	de_CH
dc.language.iso	en	de_CH
dc.publisher	MDPI	de_CH
dc.relation.ispartof	Pathogens	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Phytobacter diazotrophicus	de_CH
dc.subject	Colonization-resistance	de_CH
dc.subject	Flagellin FliC	de_CH
dc.subject	C. elegans	de_CH
dc.subject	Bacillus nematocida B16	de_CH
dc.subject.ddc	579: Mikrobiologie	de_CH
dc.title	Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.3390/pathogens11010082	de_CH
dc.identifier.doi	10.21256/zhaw-23927	-
zhaw.funding.eu	No	de_CH
zhaw.issue	1	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.start	82	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	11	de_CH
zhaw.publication.review	Open peer review	de_CH
zhaw.webfeed	Umweltgenomik	de_CH
zhaw.funding.zhaw	Antibiotic resistances in Enterobacteria	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Niu, Q., Liu, S., Yin, M., Lei, S., Rezzonico, F., & Zhang, L. (2022). Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor. Pathogens, 11(1), 82. https://doi.org/10.3390/pathogens11010082

Niu, Q. et al. (2022) ‘Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor’, Pathogens, 11(1), p. 82. Available at: https://doi.org/10.3390/pathogens11010082.

Q. Niu, S. Liu, M. Yin, S. Lei, F. Rezzonico, and L. Zhang, “Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor,” Pathogens, vol. 11, no. 1, p. 82, Jan. 2022, doi: 10.3390/pathogens11010082.

NIU, Qiuhong, Suyao LIU, Mingshen YIN, Shengwei LEI, Fabio REZZONICO und Lin ZHANG, 2022. Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor. Pathogens. 10 Januar 2022. Bd. 11, Nr. 1, S. 82. DOI 10.3390/pathogens11010082

Niu, Qiuhong, Suyao Liu, Mingshen Yin, Shengwei Lei, Fabio Rezzonico, and Lin Zhang. 2022. “Phytobacter Diazotrophicus from Intestine of Caenorhabditis Elegans Confers Colonization-Resistance against Bacillus Nematocida Using Flagellin (FliC) as an Inhibition Factor.” Pathogens 11 (1): 82. https://doi.org/10.3390/pathogens11010082.

Niu, Qiuhong, et al. “Phytobacter Diazotrophicus from Intestine of Caenorhabditis Elegans Confers Colonization-Resistance against Bacillus Nematocida Using Flagellin (FliC) as an Inhibition Factor.” Pathogens, vol. 11, no. 1, Jan. 2022, p. 82, https://doi.org/10.3390/pathogens11010082.