Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables

Witte, Sander; Huijboom, Linda; Klamert, Silvia; van de Straat, Leoni; Hagens, Steven; Fieseler, Lars; de Vegt, Bert T.; van Mierlo, Joël T.

doi:10.1016/j.fm.2022.103978

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables
Authors:	Witte, Sander Huijboom, Linda Klamert, Silvia van de Straat, Leoni Hagens, Steven Fieseler, Lars de Vegt, Bert T. van Mierlo, Joël T.
et. al:	No
DOI:	10.1016/j.fm.2022.103978
Published in:	Food Microbiology
Volume(Issue):	104
Issue:	103978
Issue Date:	Jun-2022
Publisher / Ed. Institution:	Elsevier
ISSN:	0740-0020 1095-9998
Language:	English
Subjects:	Escherichia coli; STEC; Bacteriophage; Phage; Beef meat; Romaine lettuce; Vegetables
Subject (DDC):	579: Microbiology 664: Food technology
Abstract:	Shiga toxin producing Escherichia coli (STEC) are common etiological agents of food borne illnesses and outbreaks, most often caused by consuming contaminated beef products, followed by raw vegetables and dairy products. Patients infected with E. coli O157 are more likely hospitalized than patients infected with non-O157 STEC, making E. coli O157 an important target for microbiological interventions. We show that a cocktail of bacteriophages EP75 and EP335 effectively reduces E. coli O157 on beef, romaine lettuce, spinach, and zucchini. Treatment of contaminated beef with either 2 × 107 or 1 × 108 PFU/cm2 of bacteriophage cocktail EP75/EP335 resulted in reductions of 0.8–1.1 log10 CFU/cm2 and 0.9–1.3 log10 CFU/cm2, respectively (P < 0.0001). Similarly, bacteriophage treatments of contaminated romaine lettuce, zucchini, or spinach showed significant (P < 0.05) E. coli O157 reductions of 0.7–1.9 log10 CFU/cm2 (2 × 107 PFU/cm2), and 1.4–2.4 log10 CFU/cm2 (1 × 108 PFU/cm2). An E. coli O157 reduction of 0.9 log10 and 2.0 log10 was observed already 30 min after phage application of 1 × 108 PFU/cm2 on beef and romaine lettuce, respectively. These data show that bacteriophages EP75 and EP335 can be effectively used as a processing aid on beef and vegetables, and thereby can aid industry to reduce the risk of E. coli O157 food poisoning.
URI:	https://digitalcollection.zhaw.ch/handle/11475/24457
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Food and Beverage Innovation (ILGI)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Witte, S., Huijboom, L., Klamert, S., van de Straat, L., Hagens, S., Fieseler, L., de Vegt, B. T., & van Mierlo, J. T. (2022). Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables. Food Microbiology, 104(103978). https://doi.org/10.1016/j.fm.2022.103978

Witte, S. et al. (2022) ‘Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables’, Food Microbiology, 104(103978). Available at: https://doi.org/10.1016/j.fm.2022.103978.

S. Witte et al., “Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables,” Food Microbiology, vol. 104, no. 103978, Jun. 2022, doi: 10.1016/j.fm.2022.103978.

WITTE, Sander, Linda HUIJBOOM, Silvia KLAMERT, Leoni VAN DE STRAAT, Steven HAGENS, Lars FIESELER, Bert T. DE VEGT und Joël T. VAN MIERLO, 2022. Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables. Food Microbiology. Juni 2022. Bd. 104, Nr. 103978. DOI 10.1016/j.fm.2022.103978

Witte, Sander, Linda Huijboom, Silvia Klamert, Leoni van de Straat, Steven Hagens, Lars Fieseler, Bert T. de Vegt, and Joël T. van Mierlo. 2022. “Application of Bacteriophages EP75 and EP335 Efficiently Reduces Viable Cell Counts of Escherichia Coli O157 on Beef and Vegetables.” Food Microbiology 104 (103978). https://doi.org/10.1016/j.fm.2022.103978.

Witte, Sander, et al. “Application of Bacteriophages EP75 and EP335 Efficiently Reduces Viable Cell Counts of Escherichia Coli O157 on Beef and Vegetables.” Food Microbiology, vol. 104, no. 103978, June 2022, https://doi.org/10.1016/j.fm.2022.103978.