Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Papadopoulou, Athena | - |
dc.contributor.author | Peters, Christin | - |
dc.contributor.author | Borchert, Sonja | - |
dc.contributor.author | Steiner, Kerstin | - |
dc.contributor.author | Buller, Rebecca | - |
dc.date.accessioned | 2022-07-15T14:23:57Z | - |
dc.date.available | 2022-07-15T14:23:57Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 1083-6160 | de_CH |
dc.identifier.issn | 1520-586X | de_CH |
dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/25300 | - |
dc.description.abstract | Ene reductases catalyze the biocatalytic reduction of activated alkenes, offering a powerful biobased alternative to metal-catalyzed and organocatalyzed double-bond reductions. With the aim to utilize the natural catalysts for the development of sustainable industrial processes for the flavor and fragrance (F&F) industry, we investigated the synthetic potential of a wild-type ene reductase library consisting of 20 enzymes to produce flavor compounds, including decanal. In our library screening, we identified several ene reductases that could efficiently reduce 2E-decenal as well as other investigated target substrates. Five of the characterized enzymes exhibited high reduction activities even at increased substrate concentrations (10 g/L). By analyzing additional enzyme characteristics (thermostability, solubility, and activity at 10 °C), enzyme Pbr-ER from Pseudomonas brassicacearum was chosen for further characterization and process optimization. Using optimized reaction conditions, the Pbr-ER-catalyzed reduction of 2Edecenal was performed at 100 mL scale at 40 g/L substrate concentration, achieving a high conversion yield (>93%) within 24 h. | de_CH |
dc.language.iso | en | de_CH |
dc.publisher | American Chemical Society | de_CH |
dc.relation.ispartof | Organic Process Research & Development | de_CH |
dc.rights | Licence according to publishing contract | de_CH |
dc.subject | Old yellow enzyme | de_CH |
dc.subject | Flavor compound | de_CH |
dc.subject | Industrial biocatalysis | de_CH |
dc.subject | Ene reductase | de_CH |
dc.subject | Double-bond reduction | de_CH |
dc.subject.ddc | 660: Technische Chemie | de_CH |
dc.title | Development of an ene reductase-based biocatalytic process for the production of flavor compounds | de_CH |
dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
dcterms.type | Text | de_CH |
zhaw.departement | Life Sciences und Facility Management | de_CH |
zhaw.organisationalunit | Institut für Chemie und Biotechnologie (ICBT) | de_CH |
dc.identifier.doi | 10.1021/acs.oprd.2c00096 | de_CH |
zhaw.funding.eu | No | de_CH |
zhaw.issue | 7 | de_CH |
zhaw.originated.zhaw | Yes | de_CH |
zhaw.pages.end | 2110 | de_CH |
zhaw.pages.start | 2102 | de_CH |
zhaw.publication.status | publishedVersion | de_CH |
zhaw.volume | 26 | de_CH |
zhaw.publication.review | Peer review (Publikation) | de_CH |
zhaw.webfeed | Biokatalyse | de_CH |
zhaw.author.additional | No | de_CH |
zhaw.display.portrait | Yes | de_CH |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Papadopoulou, A., Peters, C., Borchert, S., Steiner, K., & Buller, R. (2022). Development of an ene reductase-based biocatalytic process for the production of flavor compounds. Organic Process Research & Development, 26(7), 2102–2110. https://doi.org/10.1021/acs.oprd.2c00096
Papadopoulou, A. et al. (2022) ‘Development of an ene reductase-based biocatalytic process for the production of flavor compounds’, Organic Process Research & Development, 26(7), pp. 2102–2110. Available at: https://doi.org/10.1021/acs.oprd.2c00096.
A. Papadopoulou, C. Peters, S. Borchert, K. Steiner, and R. Buller, “Development of an ene reductase-based biocatalytic process for the production of flavor compounds,” Organic Process Research & Development, vol. 26, no. 7, pp. 2102–2110, 2022, doi: 10.1021/acs.oprd.2c00096.
PAPADOPOULOU, Athena, Christin PETERS, Sonja BORCHERT, Kerstin STEINER und Rebecca BULLER, 2022. Development of an ene reductase-based biocatalytic process for the production of flavor compounds. Organic Process Research & Development. 2022. Bd. 26, Nr. 7, S. 2102–2110. DOI 10.1021/acs.oprd.2c00096
Papadopoulou, Athena, Christin Peters, Sonja Borchert, Kerstin Steiner, and Rebecca Buller. 2022. “Development of an Ene Reductase-Based Biocatalytic Process for the Production of Flavor Compounds.” Organic Process Research & Development 26 (7): 2102–10. https://doi.org/10.1021/acs.oprd.2c00096.
Papadopoulou, Athena, et al. “Development of an Ene Reductase-Based Biocatalytic Process for the Production of Flavor Compounds.” Organic Process Research & Development, vol. 26, no. 7, 2022, pp. 2102–10, https://doi.org/10.1021/acs.oprd.2c00096.
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