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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25306
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dc.contributor.authorMüller, Jan-
dc.contributor.authorOtt, Vivian-
dc.contributor.authorEibl, Dieter-
dc.contributor.authorEibl-Schindler, Regine-
dc.date.accessioned2022-07-15T14:48:05Z-
dc.date.available2022-07-15T14:48:05Z-
dc.date.issued2022-
dc.identifier.issn2227-9717de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/25306-
dc.description.abstractA current focus of biopharmaceutical research and production is seed train process intensification. This allows for intermediate cultivation steps to be avoided or even for the direct inoculation of a production bioreactor with cells from cryovials or cryobags. Based on preliminary investigations regarding the suitability of high cell densities for cryopreservation and the suitability of cells from perfusion cultivations as inoculum for further cultivations, an ultra-high cell density working cell bank (UHCD-WCB) was established for an immunoglobulin G (IgG)-producing Chinese hamster ovary (CHO) cell line. The cells were previously expanded in a wave-mixed bioreactor with internal filter-based perfusion and a 1 L working volume. This procedure allows for cryovial freezing at 260 × 10<sup>6</sup> cells mL<sup>−1</sup> for the first time. The cryovials are suitable for the direct inoculation of N−1 bioreactors in the perfusion mode. These in turn can be used to inoculate subsequent IgG productions in the fed-batch mode (low-seed fed-batch or high-seed fed-batch) or the continuous mode. A comparison with the standard approach shows that cell growth and antibody production are comparable, but time savings of greater than 35% are possible for inoculum production.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofProcessesde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectChinese hamster ovary cellde_CH
dc.subjectCryopreservationde_CH
dc.subjectMonoclonal antibodyde_CH
dc.subjectN−1 perfusionde_CH
dc.subjectProcess intensificationde_CH
dc.subjectUpstream processingde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleSeed train intensification using an ultra-high cell density cell banking processde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/pr10050911de_CH
dc.identifier.doi10.21256/zhaw-25306-
zhaw.funding.euNode_CH
zhaw.issue5de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start911de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
zhaw.monitoring.costperiod2022de_CH
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Müller, J., Ott, V., Eibl, D., & Eibl-Schindler, R. (2022). Seed train intensification using an ultra-high cell density cell banking process. Processes, 10(5), 911. https://doi.org/10.3390/pr10050911
Müller, J. et al. (2022) ‘Seed train intensification using an ultra-high cell density cell banking process’, Processes, 10(5), p. 911. Available at: https://doi.org/10.3390/pr10050911.
J. Müller, V. Ott, D. Eibl, and R. Eibl-Schindler, “Seed train intensification using an ultra-high cell density cell banking process,” Processes, vol. 10, no. 5, p. 911, 2022, doi: 10.3390/pr10050911.
MÜLLER, Jan, Vivian OTT, Dieter EIBL und Regine EIBL-SCHINDLER, 2022. Seed train intensification using an ultra-high cell density cell banking process. Processes. 2022. Bd. 10, Nr. 5, S. 911. DOI 10.3390/pr10050911
Müller, Jan, Vivian Ott, Dieter Eibl, and Regine Eibl-Schindler. 2022. “Seed Train Intensification Using an Ultra-High Cell Density Cell Banking Process.” Processes 10 (5): 911. https://doi.org/10.3390/pr10050911.
Müller, Jan, et al. “Seed Train Intensification Using an Ultra-High Cell Density Cell Banking Process.” Processes, vol. 10, no. 5, 2022, p. 911, https://doi.org/10.3390/pr10050911.


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