Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature

Chatzidimitriou, Evangelia; Bisaccia, Paola; Corrà, Francesca; Bonato, Marco; Irato, Paola; Manuto, Laura; Toppo, Stefano; Bakiu, Rigers; Santovito, Gianfranco

doi:10.3390/antiox9040325

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26000

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature
Authors:	Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco
et. al:	No
DOI:	10.3390/antiox9040325 10.21256/zhaw-26000
Published in:	Antioxidants
Volume(Issue):	9
Issue:	4
Page(s):	325
Issue Date:	2020
Publisher / Ed. Institution:	MDPI
ISSN:	2076-3921
Language:	English
Subjects:	Antarctica; Chionodraco hamatus; Cold adaptation; Gene expression; Molecular evolution; Protein purification; Superoxide dismutase; Teleost
Subject (DDC):	572: Biochemistry 590: Animals (Zoology)
Abstract:	In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the "unclock-like" behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26000
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Chatzidimitriou, E., Bisaccia, P., Corrà, F., Bonato, M., Irato, P., Manuto, L., Toppo, S., Bakiu, R., & Santovito, G. (2020). Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature. Antioxidants, 9(4), 325. https://doi.org/10.3390/antiox9040325

Chatzidimitriou, E. et al. (2020) ‘Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature’, Antioxidants, 9(4), p. 325. Available at: https://doi.org/10.3390/antiox9040325.

E. Chatzidimitriou et al., “Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature,” Antioxidants, vol. 9, no. 4, p. 325, 2020, doi: 10.3390/antiox9040325.

CHATZIDIMITRIOU, Evangelia, Paola BISACCIA, Francesca CORRÀ, Marco BONATO, Paola IRATO, Laura MANUTO, Stefano TOPPO, Rigers BAKIU und Gianfranco SANTOVITO, 2020. Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature. Antioxidants. 2020. Bd. 9, Nr. 4, S. 325. DOI 10.3390/antiox9040325

Chatzidimitriou, Evangelia, Paola Bisaccia, Francesca Corrà, Marco Bonato, Paola Irato, Laura Manuto, Stefano Toppo, Rigers Bakiu, and Gianfranco Santovito. 2020. “Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco Hamatus : Antioxidant Defense at Constant Sub-Zero Temperature.” Antioxidants 9 (4): 325. https://doi.org/10.3390/antiox9040325.

Chatzidimitriou, Evangelia, et al. “Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco Hamatus : Antioxidant Defense at Constant Sub-Zero Temperature.” Antioxidants, vol. 9, no. 4, 2020, p. 325, https://doi.org/10.3390/antiox9040325.