Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material

Knobloch, Marco C.; Schinkel, Lena; Kohler, Hans-Peter E.; Mathis, Flurin; Kern, Susanne; Bleiner, Davide; Heeb, Norbert V.

doi:10.1016/j.chemosphere.2021.131199

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26804

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DC Field	Value	Language
dc.contributor.author	Knobloch, Marco C.	-
dc.contributor.author	Schinkel, Lena	-
dc.contributor.author	Kohler, Hans-Peter E.	-
dc.contributor.author	Mathis, Flurin	-
dc.contributor.author	Kern, Susanne	-
dc.contributor.author	Bleiner, Davide	-
dc.contributor.author	Heeb, Norbert V.	-
dc.date.accessioned	2023-02-06T15:06:54Z	-
dc.date.available	2023-02-06T15:06:54Z	-
dc.date.issued	2021	-
dc.identifier.issn	0045-6535	de_CH
dc.identifier.issn	1879-1298	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/26804	-
dc.description.abstract	Structure, reactivity and physico-chemical properties of polyhalogenated compounds determine their up-take, transport, bio-accumulation, transformation and toxicity and their environmental fate. In technical mixtures of chlorinated paraffins (CPs), these properties are distributed due to the presence of thousands of homologues. We hypothesized that roles of CP dehalogenation reactions, catalyzed by the haloalkane dehalogenase LinB, depend on structural properties of the substrates, e.g. chlorination degree and carbon-chain length. We exposed mixtures of chlorinated undecanes, dodecanes and tridecanes in-vitro to LinB from Sphingobium Indicum bacteria. These single-chain CP-materials also contain small amounts of chlorinated olefins (COs), which can be distinct by mathematical deconvolution of respective mass-spectra. With this procedure, we obtained homologue-specific transformation kinetics of substrates differing in saturation degree, chlorination degree and carbon chain-length. For all homologues, two-stage first-order kinetic models were established, which described the faster conversion of reactive material and the slower transformation of more persistent material. Half-lifes of 0.5-3.2 h and 56-162 h were determined for more reactive and more persistent CP-material. Proportions of persistent material increased steadily from 18 to 67% for lower (Cl6) to higher (Cl11) chlorinated paraffins and olefins. Conversion efficiencies decreased with increasing chlorination degree from 97 to 70%. Carbon-chain length had only minor effects on transformation rates. Hence, the conversion was faster and more efficient for lower-chlorinated material, and slower for higher-chlorinated and longer-chained CPs and COs. Current legislation has banned short-chain chlorinated paraffins (SCCPs) and forced a transition to longer-chain CPs. This may be counterproductive with regard to enzymatic transformation with LinB.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Elsevier	de_CH
dc.relation.ispartof	Chemosphere	de_CH
dc.rights	https://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Chlorinated olefins (COs)	de_CH
dc.subject	Chlorinated paraffins (CPs)	de_CH
dc.subject	Enzymatic dechlorination	de_CH
dc.subject	First-order kinetic model	de_CH
dc.subject	Persistent organic pollutants (POPs)	de_CH
dc.subject	Alkenes	de_CH
dc.subject	Environmental Monitoring	de_CH
dc.subject	Kinetics	de_CH
dc.subject	Paraffin	de_CH
dc.subject	Hydrocarbons, Chlorinated	de_CH
dc.subject	Sphingomonadaceae	de_CH
dc.subject.ddc	660: Technische Chemie	de_CH
dc.title	Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Chemie und Biotechnologie (ICBT)	de_CH
dc.identifier.doi	10.1016/j.chemosphere.2021.131199	de_CH
dc.identifier.doi	10.21256/zhaw-26804	-
dc.identifier.pmid	34153917	de_CH
zhaw.funding.eu	No	de_CH
zhaw.issue	131199	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	283	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Knobloch, M. C., Schinkel, L., Kohler, H.-P. E., Mathis, F., Kern, S., Bleiner, D., & Heeb, N. V. (2021). Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material. Chemosphere, 283(131199). https://doi.org/10.1016/j.chemosphere.2021.131199

Knobloch, M.C. et al. (2021) ‘Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material’, Chemosphere, 283(131199). Available at: https://doi.org/10.1016/j.chemosphere.2021.131199.

M. C. Knobloch et al., “Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material,” Chemosphere, vol. 283, no. 131199, 2021, doi: 10.1016/j.chemosphere.2021.131199.

KNOBLOCH, Marco C., Lena SCHINKEL, Hans-Peter E. KOHLER, Flurin MATHIS, Susanne KERN, Davide BLEINER und Norbert V. HEEB, 2021. Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum : Kinetic models for the homologue-specific conversion of reactive and persistent material. Chemosphere. 2021. Bd. 283, Nr. 131199. DOI 10.1016/j.chemosphere.2021.131199

Knobloch, Marco C., Lena Schinkel, Hans-Peter E. Kohler, Flurin Mathis, Susanne Kern, Davide Bleiner, and Norbert V. Heeb. 2021. “Transformation of Short-Chain Chlorinated Paraffins and Olefins with the Bacterial Dehalogenase LinB from Sphingobium Indicum : Kinetic Models for the Homologue-Specific Conversion of Reactive and Persistent Material.” Chemosphere 283 (131199). https://doi.org/10.1016/j.chemosphere.2021.131199.

Knobloch, Marco C., et al. “Transformation of Short-Chain Chlorinated Paraffins and Olefins with the Bacterial Dehalogenase LinB from Sphingobium Indicum : Kinetic Models for the Homologue-Specific Conversion of Reactive and Persistent Material.” Chemosphere, vol. 283, no. 131199, 2021, https://doi.org/10.1016/j.chemosphere.2021.131199.