Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase

Heeb, Norbert V.; Schalles, Simone; Lehner, Sandro; Schinkel, Lena; Schilling, Iris; Lienemann, Peter; Bogdal, Christian; Kohler, Hans-Peter E.

doi:10.1016/j.chemosphere.2019.03.169

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase
Authors:	Heeb, Norbert V. Schalles, Simone Lehner, Sandro Schinkel, Lena Schilling, Iris Lienemann, Peter Bogdal, Christian Kohler, Hans-Peter E.
et. al:	No
DOI:	10.1016/j.chemosphere.2019.03.169
Published in:	Chemosphere
Volume(Issue):	226
Page(s):	744
Pages to:	754
Issue Date:	Jul-2019
Publisher / Ed. Institution:	Elsevier
ISSN:	0045-6535 1879-1298
Language:	English
Subjects:	Bimodal first-order kinetics; Chlorinated olefins; Chlorinated paraffins; Deconvolution of mass spectra; Enzymatic transformation; Biotransformation; Environmental Monitoring; Halogenation; Hexachlorocyclohexane; Hydrocarbons, Brominated; Hydrolase; Paraffin
Subject (DDC):	660: Chemical engineering
Abstract:	Short-chain chlorinated paraffins (SCCPs) are polyhalogenated hydrocarbons as are hexachlorocyclohexanes (HCHs) and hexabromocyclododecanes (HBCDs). They all have been classified as persistent organic pollutants (POPs) under the UN Stockholm Convention. Per se such compounds are transformed slowly in the environment, transported over long distances and accumulate in biota. Several Sphingomonadacea strains isolated from HCH dump sites have evolved to express enzymes that can transform HCHs and HBCDs. We hypothesized that LinA2, a dehydrohalogenase expressed in such bacteria, may also transform CPs to chlorinated olefins (COs). Three mixtures of penta- to deca-chlorinated undecanes (C11), dodecanes (C12) and tridecanes (C13) were exposed to LinA2. High-resolution full-scan mass spectra (R∼8'000) of CPs and COs were obtained applying a soft ionization method, enhancing chloride-adduct [M+Cl]- formation. A mathematical deconvolution procedure was used to separate interfering spectra to verify that LinA2 indeed catalyzed the conversion of CPs to COs. About 20-40% of the material was transformed in 24 h, about 50-70% was converted in 200 h. A bimodal first-order kinetic model could describe transformations of reactive and persistent CPs. Under the given conditions reactive CPs (τ1/2 = 1.4-6.9 h) were converted 30 to 190-times faster than the persistent ones (τ1/2 = 150-260 h). Proportions of persistent isomers (pp) varied from 60 to 80%. Lower chlorinated homologues contained higher proportions of persistent isomers. In conclusion, SCCP mixtures contain both, material that is readily converted by LinA2, and persistent material that is not or only slowly transformed.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26806
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Heeb, N. V., Schalles, S., Lehner, S., Schinkel, L., Schilling, I., Lienemann, P., Bogdal, C., & Kohler, H.-P. E. (2019). Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase. Chemosphere, 226, 744–754. https://doi.org/10.1016/j.chemosphere.2019.03.169

Heeb, N.V. et al. (2019) ‘Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase’, Chemosphere, 226, pp. 744–754. Available at: https://doi.org/10.1016/j.chemosphere.2019.03.169.

N. V. Heeb et al., “Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase,” Chemosphere, vol. 226, pp. 744–754, Jul. 2019, doi: 10.1016/j.chemosphere.2019.03.169.

HEEB, Norbert V., Simone SCHALLES, Sandro LEHNER, Lena SCHINKEL, Iris SCHILLING, Peter LIENEMANN, Christian BOGDAL und Hans-Peter E. KOHLER, 2019. Biotransformation of short-chain chlorinated paraffins (SCCPs) with LinA2 : A HCH and HBCD converting bacterial dehydrohalogenase. Chemosphere. Juli 2019. Bd. 226, S. 744–754. DOI 10.1016/j.chemosphere.2019.03.169

Heeb, Norbert V., Simone Schalles, Sandro Lehner, Lena Schinkel, Iris Schilling, Peter Lienemann, Christian Bogdal, and Hans-Peter E. Kohler. 2019. “Biotransformation of Short-Chain Chlorinated Paraffins (SCCPs) with LinA2 : A HCH and HBCD Converting Bacterial Dehydrohalogenase.” Chemosphere 226 (July): 744–54. https://doi.org/10.1016/j.chemosphere.2019.03.169.

Heeb, Norbert V., et al. “Biotransformation of Short-Chain Chlorinated Paraffins (SCCPs) with LinA2 : A HCH and HBCD Converting Bacterial Dehydrohalogenase.” Chemosphere, vol. 226, July 2019, pp. 744–54, https://doi.org/10.1016/j.chemosphere.2019.03.169.