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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26856
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dc.contributor.authorSpäter, Thomas-
dc.contributor.authorAssunção, Marisa-
dc.contributor.authorLit, Kwok Keung-
dc.contributor.authorGong, Guidong-
dc.contributor.authorWang, Xiaoling-
dc.contributor.authorChen, Yi-Yun-
dc.contributor.authorRao, Ying-
dc.contributor.authorLi, Yucong-
dc.contributor.authorYiu, Chi Him Kendrick-
dc.contributor.authorLaschke, Matthias W.-
dc.contributor.authorMenger, Michael D.-
dc.contributor.authorWang, Dan-
dc.contributor.authorTuan, Rocky S.-
dc.contributor.authorKhoo, Kay-Hooi-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorGuo, Junling-
dc.contributor.authorBlocki, Anna-
dc.date.accessioned2023-02-09T12:02:31Z-
dc.date.available2023-02-09T12:02:31Z-
dc.date.issued2022-
dc.identifier.issn2097-1192de_CH
dc.identifier.issn2452-199Xde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/26856-
dc.description.abstractTissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cultures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the therapeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches.de_CH
dc.language.isoende_CH
dc.publisherKeAide_CH
dc.relation.ispartofBioactive Materialsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectDextran sulfatede_CH
dc.subjectExtracellular matrixde_CH
dc.subjectMesenchymal stem cellde_CH
dc.subjectPoly-electrolyte-driven co-assemblyde_CH
dc.subjectTherapeutic angiogenesisde_CH
dc.subjectWound healingde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleEngineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesisde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.bioactmat.2022.03.015de_CH
dc.identifier.doi10.21256/zhaw-26856-
dc.identifier.pmid35846945de_CH
zhaw.funding.euNode_CH
zhaw.issue17de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end541de_CH
zhaw.pages.start526de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Später, T., Assunção, M., Lit, K. K., Gong, G., Wang, X., Chen, Y.-Y., Rao, Y., Li, Y., Yiu, C. H. K., Laschke, M. W., Menger, M. D., Wang, D., Tuan, R. S., Khoo, K.-H., Raghunath, M., Guo, J., & Blocki, A. (2022). Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis. Bioactive Materials, 17, 526–541. https://doi.org/10.1016/j.bioactmat.2022.03.015
Später, T. et al. (2022) ‘Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis’, Bioactive Materials, (17), pp. 526–541. Available at: https://doi.org/10.1016/j.bioactmat.2022.03.015.
T. Später et al., “Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis,” Bioactive Materials, no. 17, pp. 526–541, 2022, doi: 10.1016/j.bioactmat.2022.03.015.
SPÄTER, Thomas, Marisa ASSUNÇÃO, Kwok Keung LIT, Guidong GONG, Xiaoling WANG, Yi-Yun CHEN, Ying RAO, Yucong LI, Chi Him Kendrick YIU, Matthias W. LASCHKE, Michael D. MENGER, Dan WANG, Rocky S. TUAN, Kay-Hooi KHOO, Michael RAGHUNATH, Junling GUO und Anna BLOCKI, 2022. Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis. Bioactive Materials. 2022. Nr. 17, S. 526–541. DOI 10.1016/j.bioactmat.2022.03.015
Später, Thomas, Marisa Assunção, Kwok Keung Lit, Guidong Gong, Xiaoling Wang, Yi-Yun Chen, Ying Rao, et al. 2022. “Engineering Microparticles Based on Solidified Stem Cell Secretome with an Augmented Pro-Angiogenic Factor Portfolio for Therapeutic Angiogenesis.” Bioactive Materials, no. 17: 526–41. https://doi.org/10.1016/j.bioactmat.2022.03.015.
Später, Thomas, et al. “Engineering Microparticles Based on Solidified Stem Cell Secretome with an Augmented Pro-Angiogenic Factor Portfolio for Therapeutic Angiogenesis.” Bioactive Materials, no. 17, 2022, pp. 526–41, https://doi.org/10.1016/j.bioactmat.2022.03.015.


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