Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes

Müller, Jan; Ott, Vivian; Weiss, Noémi; Neubauer, Peter; Eibl, Dieter; Eibl, Regine

doi:10.1002/cite.202200097

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26862

Full metadata record

DC Field	Value	Language
dc.contributor.author	Müller, Jan	-
dc.contributor.author	Ott, Vivian	-
dc.contributor.author	Weiss, Noémi	-
dc.contributor.author	Neubauer, Peter	-
dc.contributor.author	Eibl, Dieter	-
dc.contributor.author	Eibl, Regine	-
dc.date.accessioned	2023-02-09T13:41:04Z	-
dc.date.available	2023-02-09T13:41:04Z	-
dc.date.issued	2022-10-17	-
dc.identifier.issn	0009-286X	de_CH
dc.identifier.issn	1522-2640	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/26862	-
dc.description.abstract	Various approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one-step inoculum production was established based on an ultra-high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1-L wave-mixed perfusion bioreactors. When reaching around 180 · 106 cells mL−1 in N−1 perfusion mode, low-seed and high-seed fed-batch experiments in shake flasks were inoculated, as was the case for 250-mL stirred single-use bioreactors. Additionally, proof-of-concept runs at 50-L and 200-L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Wiley	de_CH
dc.relation.ispartof	Chemie Ingenieur Technik	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Chinese hamster ovary cell	de_CH
dc.subject	Monoclonal antibody	de_CH
dc.subject	N-1 perfusion	de_CH
dc.subject	Ultra-high cell density	de_CH
dc.subject.ddc	660.6: Biotechnologie	de_CH
dc.title	Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Chemie und Biotechnologie (ICBT)	de_CH
dc.identifier.doi	10.1002/cite.202200097	de_CH
dc.identifier.doi	10.21256/zhaw-26862	-
zhaw.funding.eu	No	de_CH
zhaw.issue	12	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.end	1984	de_CH
zhaw.pages.start	1977	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	94	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Müller, J., Ott, V., Weiss, N., Neubauer, P., Eibl, D., & Eibl, R. (2022). Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes. Chemie Ingenieur Technik, 94(12), 1977–1984. https://doi.org/10.1002/cite.202200097

Müller, J. et al. (2022) ‘Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes’, Chemie Ingenieur Technik, 94(12), pp. 1977–1984. Available at: https://doi.org/10.1002/cite.202200097.

J. Müller, V. Ott, N. Weiss, P. Neubauer, D. Eibl, and R. Eibl, “Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes,” Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1977–1984, Oct. 2022, doi: 10.1002/cite.202200097.

MÜLLER, Jan, Vivian OTT, Noémi WEISS, Peter NEUBAUER, Dieter EIBL und Regine EIBL, 2022. Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes. Chemie Ingenieur Technik. 17 Oktober 2022. Bd. 94, Nr. 12, S. 1977–1984. DOI 10.1002/cite.202200097

Müller, Jan, Vivian Ott, Noémi Weiss, Peter Neubauer, Dieter Eibl, and Regine Eibl. 2022. “Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes.” Chemie Ingenieur Technik 94 (12): 1977–84. https://doi.org/10.1002/cite.202200097.

Müller, Jan, et al. “Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes.” Chemie Ingenieur Technik, vol. 94, no. 12, Oct. 2022, pp. 1977–84, https://doi.org/10.1002/cite.202200097.