1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26891
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRampin, Andrea-
dc.contributor.authorSkoufos, Ioannis-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorTzora, Athina-
dc.contributor.authorDiakakis, Nikolaos-
dc.contributor.authorPrassinos, Nikitas-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.date.accessioned2023-02-09T15:11:02Z-
dc.date.available2023-02-09T15:11:02Z-
dc.date.issued2022-05-05-
dc.identifier.issn2073-4409de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/26891-
dc.description.abstractThe absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed the influence of different sera (equine serum and foetal bovine serum) on equine tenocyte morphology, viability, metabolic activity, proliferation and protein synthesis as a function of tissue-specific extracellular matrix deposition (induced via macromolecular crowding), aging (passages 3, 6, 9) and time in culture (days 3, 5, 7). In comparison to cells at passage 3, at day 3, in foetal bovine serum and without macromolecular crowding (traditional equine tenocyte culture), the highest number of significantly decreased readouts were observed for cells in foetal bovine serum, at passage 3, at day 5 and day 7 and without macromolecular crowding. Again, in comparison to traditional equine tenocyte culture, the highest number of significantly increased readouts were observed for cells in equine serum, at passage 3 and passage 6, at day 7 and with macromolecular crowding. Our data advocate the use of an allogeneic serum and tissue-specific extracellular matrix for effective expansion of equine tenocytes.de_CH
dc.language.isoende_CH
dc.publisherMDPIde_CH
dc.relation.ispartofCellsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectAllogeneic serumde_CH
dc.subjectExcluded volume effectde_CH
dc.subjectFoetal bovine serumde_CH
dc.subjectSerial passagingde_CH
dc.subjectTenocyte agingde_CH
dc.subjectTenocyte functionde_CH
dc.subjectAnimalde_CH
dc.subjectExtracellular matrixde_CH
dc.subjectHorsede_CH
dc.subjectMacromolecular substancede_CH
dc.subjectSerum albumin, bovinede_CH
dc.subjectHematopoietic stem cell transplantationde_CH
dc.subjectTenocytede_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleAllogeneic serum and macromolecular crowding maintain native equine tenocyte function in culturede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.3390/cells11091562de_CH
dc.identifier.doi10.21256/zhaw-26891-
dc.identifier.pmid35563866de_CH
zhaw.funding.euNode_CH
zhaw.issue9de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start1562de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume11de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2022_Rampin-etal_Native-equine-tenocyte-function-in-culture_Cells.pdf17.51 MBAdobe PDFThumbnail
View/Open
Show simple item record
Rampin, A., Skoufos, I., Raghunath, M., Tzora, A., Diakakis, N., Prassinos, N., & Zeugolis, D. I. (2022). Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture. Cells, 11(9), 1562. https://doi.org/10.3390/cells11091562
Rampin, A. et al. (2022) ‘Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture’, Cells, 11(9), p. 1562. Available at: https://doi.org/10.3390/cells11091562.
A. Rampin et al., “Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture,” Cells, vol. 11, no. 9, p. 1562, May 2022, doi: 10.3390/cells11091562.
RAMPIN, Andrea, Ioannis SKOUFOS, Michael RAGHUNATH, Athina TZORA, Nikolaos DIAKAKIS, Nikitas PRASSINOS und Dimitrios I. ZEUGOLIS, 2022. Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture. Cells. 5 Mai 2022. Bd. 11, Nr. 9, S. 1562. DOI 10.3390/cells11091562
Rampin, Andrea, Ioannis Skoufos, Michael Raghunath, Athina Tzora, Nikolaos Diakakis, Nikitas Prassinos, and Dimitrios I. Zeugolis. 2022. “Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.” Cells 11 (9): 1562. https://doi.org/10.3390/cells11091562.
Rampin, Andrea, et al. “Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.” Cells, vol. 11, no. 9, May 2022, p. 1562, https://doi.org/10.3390/cells11091562.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.