Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging |
Authors: | Sabani, Besmira Brand, Michael Albert, Ina Inderbitzin, Joelle Eichenseher, Fritz Schmelcher, Mathias Rohrer, Jack Riedl, Rainer Lehmann, Steffi |
et. al: | No |
DOI: | 10.1016/j.nano.2022.102607 |
Published in: | Nanomedicine: Nanotechnology, Biology and Medicine |
Volume(Issue): | 47 |
Issue: | 102607 |
Issue Date: | Jan-2023 |
Publisher / Ed. Institution: | Elsevier |
ISSN: | 1549-9634 1549-9642 |
Language: | English |
Subjects: | Drug nanocarrier; Extracellular vesicle; Structure-based drug design; Surface functionalization; Targeted drug delivery; Diagnostic imaging |
Subject (DDC): | 610.28: Biomedicine, biomedical engineering 615: Pharmacology and therapeutics |
Abstract: | Extracellular vesicles (EVs), nanovesicles released by cells to effectively exchange biological information, are gaining interest as drug delivery system. Yet, analogously to liposomes, they show short blood circulation times and accumulation in the liver and the spleen. For tissue specific delivery, EV surfaces will thus have to be functionalized. We present a novel platform for flexible modification of EVs with target-specific ligands based on the avidin-biotin system. Genetic engineering of donor cells with a glycosylphosphatidylinositol-anchored avidin (GPI-Av) construct allows the isolation of EVs displaying avidin on their surface, functionalized with any biotinylated ligand. For proof of concept, GPI-Av EVs were modified with i) a biotinylated antibody or ii) de novo designed and synthesized biotinylated ligands binding carbonic anhydrase IX (CAIX), a membrane associated enzyme overexpressed in cancer. Functionalized EVs showed specific binding and uptake by CAIX-expressing cells, demonstrating the power of the system to prepare EVs for cell-specific drug delivery. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/26903 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Sabani, B., Brand, M., Albert, I., Inderbitzin, J., Eichenseher, F., Schmelcher, M., Rohrer, J., Riedl, R., & Lehmann, S. (2023). A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging. Nanomedicine: Nanotechnology, Biology and Medicine, 47(102607). https://doi.org/10.1016/j.nano.2022.102607
Sabani, B. et al. (2023) ‘A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging’, Nanomedicine: Nanotechnology, Biology and Medicine, 47(102607). Available at: https://doi.org/10.1016/j.nano.2022.102607.
B. Sabani et al., “A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging,” Nanomedicine: Nanotechnology, Biology and Medicine, vol. 47, no. 102607, Jan. 2023, doi: 10.1016/j.nano.2022.102607.
SABANI, Besmira, Michael BRAND, Ina ALBERT, Joelle INDERBITZIN, Fritz EICHENSEHER, Mathias SCHMELCHER, Jack ROHRER, Rainer RIEDL und Steffi LEHMANN, 2023. A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging. Nanomedicine: Nanotechnology, Biology and Medicine. Januar 2023. Bd. 47, Nr. 102607. DOI 10.1016/j.nano.2022.102607
Sabani, Besmira, Michael Brand, Ina Albert, Joelle Inderbitzin, Fritz Eichenseher, Mathias Schmelcher, Jack Rohrer, Rainer Riedl, and Steffi Lehmann. 2023. “A Novel Surface Functionalization Platform to Prime Extracellular Vesicles for Targeted Therapy and Diagnostic Imaging.” Nanomedicine: Nanotechnology, Biology and Medicine 47 (102607). https://doi.org/10.1016/j.nano.2022.102607.
Sabani, Besmira, et al. “A Novel Surface Functionalization Platform to Prime Extracellular Vesicles for Targeted Therapy and Diagnostic Imaging.” Nanomedicine: Nanotechnology, Biology and Medicine, vol. 47, no. 102607, Jan. 2023, https://doi.org/10.1016/j.nano.2022.102607.
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