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dc.contributor.authorAssunção, Marisa-
dc.contributor.authorWong, Christy Wingtung-
dc.contributor.authorRichardson, Joseph J.-
dc.contributor.authorTsang, Rachel-
dc.contributor.authorBeyer, Sebastian-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorBlocki, Anna-
dc.date.accessioned2023-02-11T10:32:25Z-
dc.date.available2023-02-11T10:32:25Z-
dc.date.issued2020-
dc.identifier.issn0928-4931de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/26922-
dc.description.abstractA faithful reconstruction of the native cellular microenvironment is instrumental for tissue engineering. Macromolecular crowding (MMC) empowers cells to deposit their own extracellular matrix (ECM) in greater amounts, and thus contributes to building tissue-specific complex microenvironments in vitro. Dextran sulfate (DxS, 500 kDa), a semi-synthetic sulfated polyglucose, was shown previously at a fractional volume occupancy (FVO) of 5.2% (v/v; 100 μg/ml) to act as a potent molecular crowding agent in vitro. When added to human mesenchymal stromal cell (MSC) cultures, DxS enhanced fibronectin and collagen I deposition several-fold also at concentrations with negligible FVO (<1% v/v). In a cell-free system, incubation of culture media supplemented with fetal bovine serum (FBS), purified fibronectin or collagen I with DxS led to a co-deposition of respective components, exhibiting a similar granular pattern as observed in cell culture. Aggregation of FBS components, fibronectin or collagen I with DxS was confirmed by dynamic light scattering, where an increase in hydrodynamic radius in the respective mixtures was observed. FBS- and fibronectin aggregates could be dissociated with increasing salt concentrations, indicating electrostatic forces to be responsible for the aggregation. Conversely, collagen I-DxS aggregates increased in size with increasing ion concentration, likely caused by charge screening of collagen I, which is net negatively charged at neutral pH, thus permitting weaker intermolecular interactions to occur. The incorporation of DxS into the ECM resulted in altered ECM topography and stiffness. DxS-supplemented cultures exhibited potentiated bioactivity, such as enhanced adipogenic and especially osteogenic differentiation under inductive conditions. We propose an alternative mechanism by which DxS drives ECM deposition via aggregation, and in an independent manner from MMC. A deeper understanding of the underlying mechanism will enable optimized engineering approaches for ECM-rich tissue constructs.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofMaterials Science and Engineering C: Materials for Biological Applicationsde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectDextran sulfatede_CH
dc.subjectExtracellular matrix depositionde_CH
dc.subjectMacromolecular crowdingde_CH
dc.subjectMesenchymal stromal cellde_CH
dc.subjectMicroenvironmentde_CH
dc.subjectTissue engineeringde_CH
dc.subjectAdipogenesisde_CH
dc.subjectBone marrow cellde_CH
dc.subjectCell differentiationde_CH
dc.subjectCells, culturedde_CH
dc.subjectCollagen type Ide_CH
dc.subjectCulture mediade_CH
dc.subjectDextran sulfatede_CH
dc.subjectExtracellular matrixde_CH
dc.subjectFibronectinsde_CH
dc.subjectHumansde_CH
dc.subjectMesenchymal stem Cellde_CH
dc.subjectOsteogenesisde_CH
dc.subjectStatic clectricityde_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleMacromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effectde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.msec.2019.110280de_CH
dc.identifier.pmid31753359de_CH
zhaw.funding.euNode_CH
zhaw.issue110280de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume106de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Assunção, M., Wong, C. W., Richardson, J. J., Tsang, R., Beyer, S., Raghunath, M., & Blocki, A. (2020). Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect. Materials Science and Engineering C: Materials for Biological Applications, 106(110280). https://doi.org/10.1016/j.msec.2019.110280
Assunção, M. et al. (2020) ‘Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect’, Materials Science and Engineering C: Materials for Biological Applications, 106(110280). Available at: https://doi.org/10.1016/j.msec.2019.110280.
M. Assunção et al., “Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect,” Materials Science and Engineering C: Materials for Biological Applications, vol. 106, no. 110280, 2020, doi: 10.1016/j.msec.2019.110280.
ASSUNÇÃO, Marisa, Christy Wingtung WONG, Joseph J. RICHARDSON, Rachel TSANG, Sebastian BEYER, Michael RAGHUNATH und Anna BLOCKI, 2020. Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect. Materials Science and Engineering C: Materials for Biological Applications. 2020. Bd. 106, Nr. 110280. DOI 10.1016/j.msec.2019.110280
Assunção, Marisa, Christy Wingtung Wong, Joseph J. Richardson, Rachel Tsang, Sebastian Beyer, Michael Raghunath, and Anna Blocki. 2020. “Macromolecular Dextran Sulfate Facilitates Extracellular Matrix Deposition by Electrostatic Interaction Independent from a Macromolecular Crowding Effect.” Materials Science and Engineering C: Materials for Biological Applications 106 (110280). https://doi.org/10.1016/j.msec.2019.110280.
Assunção, Marisa, et al. “Macromolecular Dextran Sulfate Facilitates Extracellular Matrix Deposition by Electrostatic Interaction Independent from a Macromolecular Crowding Effect.” Materials Science and Engineering C: Materials for Biological Applications, vol. 106, no. 110280, 2020, https://doi.org/10.1016/j.msec.2019.110280.


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