The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents

Tsiapalis, Dimitrios; De Pieri, Andrea; Spanoudes, Kyriakos; Sallent, Ignacio; Kearns, Stephen; Kelly, Jack L.; Raghunath, Michael; Zeugolis, Dimitrios I.

doi:10.1088/1758-5090/ab6412

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents
Authors:	Tsiapalis, Dimitrios De Pieri, Andrea Spanoudes, Kyriakos Sallent, Ignacio Kearns, Stephen Kelly, Jack L. Raghunath, Michael Zeugolis, Dimitrios I.
et. al:	No
DOI:	10.1088/1758-5090/ab6412
Published in:	Biofabrication
Volume(Issue):	12
Issue:	2
Page(s):	25018
Issue Date:	2020
Publisher / Ed. Institution:	IOP Publishing
ISSN:	1758-5082 1758-5090
Language:	English
Subjects:	Aggrecan; Carrageenan; Cell, ultured; Collagen type I; Collagen type III; Down-regulation; Extracellular matrix; Extracellular matrix protein; Humans; Male; Middle aged; Oxygen; Procollagen-proline dioxygenase; Tendon; Up-regulation
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Cellular therapies play an important role in tendon tissue engineering, with tenocytes being the most prominent and potent cell population available. However, for the development of a rich extracellular matrix tenocyte-assembled tendon equivalent, prolonged in vitro culture is required, which is associated with phenotypic drift. Recapitulation of tendon tissue microenvironment in vitro with cues that enhance and accelerate extracellular matrix synthesis and deposition, whilst maintaining tenocyte phenotype, may lead to functional cell therapies. Herein, we assessed the synergistic effect of low oxygen tension (enhances extracellular matrix synthesis) and macromolecular crowding (enhances extracellular matrix deposition) in human tenocyte culture. Protein analysis demonstrated that human tenocytes at 2% oxygen tension and with 50 μg ml-1 carrageenan (macromolecular crowder used) significantly increased synthesis and deposition of collagen types I, III, V and VI. Gene analysis at day 7 illustrated that human tenocytes at 2% oxygen tension and with 50 μg ml-1 carrageenan significantly increased the expression of prolyl 4-hydroxylase subunit alpha 1, procollagen-lysine 2- oxoglutarate 5-dioxygenase 2, scleraxis, tenomodulin and elastin, whilst chondrogenic (e.g. runt-related transcription factor 2, cartilage oligomeric matrix protein, aggrecan) and osteogenic (e.g. secreted phosphoprotein 1, bone gamma-carboxyglutamate protein) trans-differentiation markers were significantly down-regulated or remained unchanged. Collectively, our data clearly illustrates the beneficial synergistic effect of low oxygen tension and macromolecular crowding in the accelerated development of tissue equivalents.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27134
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Tsiapalis, D., De Pieri, A., Spanoudes, K., Sallent, I., Kearns, S., Kelly, J. L., Raghunath, M., & Zeugolis, D. I. (2020). The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents. Biofabrication, 12(2), 25018. https://doi.org/10.1088/1758-5090/ab6412

Tsiapalis, D. et al. (2020) ‘The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents’, Biofabrication, 12(2), p. 25018. Available at: https://doi.org/10.1088/1758-5090/ab6412.

D. Tsiapalis et al., “The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents,” Biofabrication, vol. 12, no. 2, p. 25018, 2020, doi: 10.1088/1758-5090/ab6412.

TSIAPALIS, Dimitrios, Andrea DE PIERI, Kyriakos SPANOUDES, Ignacio SALLENT, Stephen KEARNS, Jack L. KELLY, Michael RAGHUNATH und Dimitrios I. ZEUGOLIS, 2020. The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents. Biofabrication. 2020. Bd. 12, Nr. 2, S. 25018. DOI 10.1088/1758-5090/ab6412

Tsiapalis, Dimitrios, Andrea De Pieri, Kyriakos Spanoudes, Ignacio Sallent, Stephen Kearns, Jack L. Kelly, Michael Raghunath, and Dimitrios I. Zeugolis. 2020. “The Synergistic Effect of Low Oxygen Tension and Macromolecular Crowding in the Development of Extracellular Matrix-Rich Tendon Equivalents.” Biofabrication 12 (2): 25018. https://doi.org/10.1088/1758-5090/ab6412.

Tsiapalis, Dimitrios, et al. “The Synergistic Effect of Low Oxygen Tension and Macromolecular Crowding in the Development of Extracellular Matrix-Rich Tendon Equivalents.” Biofabrication, vol. 12, no. 2, 2020, p. 25018, https://doi.org/10.1088/1758-5090/ab6412.