Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7

Knecht, Leandra; Heinrich, Nadine; Felder, Katja; Born, Yannick; Pelludat, Cosima; Loessner, Martin; Fieseler, Lars

Publication type:	Conference poster
Type of review:	Peer review (abstract)
Title:	Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7
Authors:	Knecht, Leandra Heinrich, Nadine Felder, Katja Born, Yannick Pelludat, Cosima Loessner, Martin Fieseler, Lars
et. al:	No
Conference details:	Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018
Issue Date:	2018
Language:	English
Subjects:	Bacteriophage; Erwinia; Cellulose
Subject (DDC):	579: Microbiology
Abstract:	The plant pathogen Erwinia amylovora is the causative agent of fire blight. One of its major virulence factors is the exopolysaccharide (EPS) capsule, which plays an important role in biofilm formation. The production of EPS leads to clogging of plant vessels and does therefore provoke disease symptoms. Although the application of streptomycin can effectively control infection, the antibiotic is banned in an increasing number of countries. Bacteriophages are a promising alternative to conventional antibiotics. For the control of Gram-negative bacteria, a phage cocktail of at least two phages targeting different phage receptors is usually applied. To identify phage receptors on the surface of E. amylovora a Tn5 transposon mutant library was screened in a high throughput. Using the N4-like phage S6 and the FelixO1-like phage M7 putative phage receptors were identified. Both, M7 and S6, were not able to lyse mutants with Tn5 insertions in the bacterial cellulose synthase operon (bcs). The Bcs-complex produces bacterial cellulose, an exopolysaccharide associated with biofilm formation. Deletion mutagenesis of the entire bcs operon and of single bcs genes, verified the importance for infection of E. amylovora with S6 and M7. Experiments with the cellulose binding dye Congo red and the finding that both phages harbour a setting of cellulases and endoglucanase further supported the interaction of S6 and M7 with cellulose. The bacteriophages might recognize different patterns either on the cellulose synthase complex, e.g. the outer membrane protein BcsC, or the cellulose cargo itself.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27149
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Food and Beverage Innovation (ILGI)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Knecht, L., Heinrich, N., Felder, K., Born, Y., Pelludat, C., Loessner, M., & Fieseler, L. (2018). Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7. Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018.

Knecht, L. et al. (2018) ‘Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7’, in Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018.

L. Knecht et al., “Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7,” in Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018, 2018.

KNECHT, Leandra, Nadine HEINRICH, Katja FELDER, Yannick BORN, Cosima PELLUDAT, Martin LOESSNER und Lars FIESELER, 2018. Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7. In: Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018. Conference poster. 2018

Knecht, Leandra, Nadine Heinrich, Katja Felder, Yannick Born, Cosima Pelludat, Martin Loessner, and Lars Fieseler. 2018. “Mutations in the Bcs Operon of Erwinia Amylovora Abolish Infection by Bacteriophages S6 and M7.” Conference poster. In Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018.

Knecht, Leandra, et al. “Mutations in the Bcs Operon of Erwinia Amylovora Abolish Infection by Bacteriophages S6 and M7.” Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018, 2018.