PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora

Kammerecker, Sandrine; Gayder, Steven; Gervásio, Kellen; Henriques, André; Torres-Cortés, Gloria; Kőrösiné Papp, Szilvia; Kovács, Tamás; Raffai, Krisztina; Cabezón Largas, Sixto; de Santos Prieto, Borja; Fieseler, Lars

Publication type:	Conference other
Type of review:	Peer review (abstract)
Title:	PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora
Authors:	Kammerecker, Sandrine Gayder, Steven Gervásio, Kellen Henriques, André Torres-Cortés, Gloria Kőrösiné Papp, Szilvia Kovács, Tamás Raffai, Krisztina Cabezón Largas, Sixto de Santos Prieto, Borja Fieseler, Lars
et. al:	No
Conference details:	Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022
Issue Date:	6-Sep-2022
Language:	English
Subjects:	Bacteriophage; Biopesticide; Formulation; UV-protection
Subject (DDC):	579: Microbiology
Abstract:	Many plant protection agents traditionally used to combat fire blight are subjected to restrictions in an increasing number of countries. The emergence of streptomycin-resistant bacteria has called for antibiotic-free alternatives, while the use of aluminum- and copper-based products has raised environmental concerns. In this scope, there is a high demand for alternative products with lower risk profiles, for example antagonistic bacteria, yeasts, or bacteriophages (phages). Phages are considered safe for both the environment and human health, and they are highly specific for their target bacterium. Some phage-based agents have already been approved for use in plant protection and food safety. However, diverse environmental factors can impair the phages’ stability. UV-light, for example, which is damaging to DNA, can inactivate phages in the field. In the European Horizon 2020 project PhageFire, the goal of all involved partners is to develop such a phage-based biopesticide against fire blight. Here, we tested different UV-B absorbing substances and surfactants to develop an effective formulation to protect the bacteriophages from UV-B light and to ensure an even foliage coverage and adherence of the phages to the plant surface. Under artificial UV-B illumination, the phages’ survival could be increased by the addition of UV-B absorbents. We could also show that many commercially available surfactants are compatible with phages and do not affect their stability. With these contributions, complemented by phage characterization, field trials, and regulatory aspects, we aim to develop an effective and safe biopesticide against fire blight.
URI:	https://digitalcollection.zhaw.ch/handle/11475/27163
Fulltext version:	Published version
License (according to publishing contract):	Not specified
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Food and Beverage Innovation (ILGI)
Published as part of the ZHAW project:	PhageFire – An effective and environmentally friendly solution to control fire blight disease caused by Erwinia amylovora in pome fruit crops
Appears in collections:	Publikationen Life Sciences und Facility Management

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Kammerecker, S., Gayder, S., Gervásio, K., Henriques, A., Torres-Cortés, G., Kőrösiné Papp, S., Kovács, T., Raffai, K., Cabezón Largas, S., de Santos Prieto, B., & Fieseler, L. (2022, September 6). PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora. Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

Kammerecker, S. et al. (2022) ‘PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora’, in Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

S. Kammerecker et al., “PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora,” in Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022, Sep. 2022.

KAMMERECKER, Sandrine, Steven GAYDER, Kellen GERVÁSIO, André HENRIQUES, Gloria TORRES-CORTÉS, Szilvia KŐRÖSINÉ PAPP, Tamás KOVÁCS, Krisztina RAFFAI, Sixto CABEZÓN LARGAS, Borja DE SANTOS PRIETO und Lars FIESELER, 2022. PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora. In: Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022. Conference presentation. 6 September 2022

Kammerecker, Sandrine, Steven Gayder, Kellen Gervásio, André Henriques, Gloria Torres-Cortés, Szilvia Kőrösiné Papp, Tamás Kovács, et al. 2022. “PhageFire : Formulation of a Bacteriophage-Based Biopesticide against Erwinia Amylovora.” Conference presentation. In Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.

Kammerecker, Sandrine, et al. “PhageFire : Formulation of a Bacteriophage-Based Biopesticide against Erwinia Amylovora.” Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022, 2022.