| Publication type: | Conference other |
| Type of review: | Peer review (abstract) |
| Title: | High-throughput analysis of microalgae cell wall permeability in biotechnological production settings |
| Authors: | Carpine, R. Fluri, M. Müller, T. Straumann, M. Neutsch, L. |
| et. al: | No |
| DOI: | 10.1016/j.nbt.2018.05.907 |
| Published in: | New Biotechnology |
| Proceedings: | Abstracts of the 18th European Congress on Biotechnology |
| Volume(Issue): | 44 |
| Issue: | Supplement |
| Page(s): | S79 |
| Conference details: | 18th European Congress on Biotechnology (ECB), Geneva, Switzerland, 1-4 July 2018 |
| Issue Date: | 2018 |
| Publisher / Ed. Institution: | Elsevier |
| ISSN: | 1876-4347 1871-6784 |
| Language: | English |
| Subjects: | Microalgae cell; Biotechnological production |
| Subject (DDC): | 579: Microbiology 660.6: Biotechnology |
| Abstract: | Microalgae represent a promising resource for nature-derived compounds in different fields of interest. The rigid microalgae cell wall (composed of a polysaccharide and glycoprotein matrix) provides the cells with a formidable defence against harsh environments, but typically poses a major bottleneck for efficient product extraction, and hence sustainable process setups. The aim of this study was to evaluate the applicability of a high-throughput screening method for microalgae cell wall permeability, based on flow cytometry and titration against the exposure time in proceeding fixation steps. A panel of comparative trials were carried out on Chlorella vulgaris cultures to visualize changes in wall permeability via different fixation and staining techniques (methanol treatment and microwaving). Different intracellular targets were addressed (lipid storage vesicles and DNA) to define the optimal strategy with regard to information content on wall configuration and overall robustness. The tests were carried out for different growth conditions (photoautotrophic and heterotrophic cultures) and process states.The main results showed that both dies efficiently reached their intracellular target depending on fixation conditions, with minimal non-specific staining. Both assay formats yielded valuable, but non-redundant information on the current wall permeability. As expected, analyses targeted to DNA content proved to be more stable over culture time than those directed to lipid depositories, which can subjected to dynamic changes in response to growth conditions.The future perspectives will be to implement the flow cytometric assays as a real-time monitoring tool to exert control over product extraction efficiency in different microalgae strains and biotechnological production settings. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/27590 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Carpine, R., Fluri, M., Müller, T., Straumann, M., & Neutsch, L. (2018). High-throughput analysis of microalgae cell wall permeability in biotechnological production settings [Conference presentation]. New Biotechnology, 44(Supplement), S79. https://doi.org/10.1016/j.nbt.2018.05.907
Carpine, R. et al. (2018) ‘High-throughput analysis of microalgae cell wall permeability in biotechnological production settings’, in New Biotechnology. Elsevier, p. S79. Available at: https://doi.org/10.1016/j.nbt.2018.05.907.
R. Carpine, M. Fluri, T. Müller, M. Straumann, and L. Neutsch, “High-throughput analysis of microalgae cell wall permeability in biotechnological production settings,” in New Biotechnology, 2018, vol. 44, no. Supplement, p. S79. doi: 10.1016/j.nbt.2018.05.907.
CARPINE, R., M. FLURI, T. MÜLLER, M. STRAUMANN und L. NEUTSCH, 2018. High-throughput analysis of microalgae cell wall permeability in biotechnological production settings. In: New Biotechnology. Conference presentation. Elsevier. 2018. S. S79
Carpine, R., M. Fluri, T. Müller, M. Straumann, and L. Neutsch. 2018. “High-Throughput Analysis of Microalgae Cell Wall Permeability in Biotechnological Production Settings.” Conference presentation. In New Biotechnology, 44:S79. Elsevier. https://doi.org/10.1016/j.nbt.2018.05.907.
Carpine, R., et al. “High-Throughput Analysis of Microalgae Cell Wall Permeability in Biotechnological Production Settings.” New Biotechnology, vol. 44, no. Supplement, Elsevier, 2018, p. S79, https://doi.org/10.1016/j.nbt.2018.05.907.
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