Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition

Koch, Franziska; Müller, Michael; König, Finja; Meyer, Nina; Gattlen, Jasmin; Pieles, Uwe; Peters, Kirsten; Kreikemeyer, Bernd; Mathes, Stephanie; Saxer, Sina

doi:10.1098/rsos.171562

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-28950

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition
Authors:	Koch, Franziska Müller, Michael König, Finja Meyer, Nina Gattlen, Jasmin Pieles, Uwe Peters, Kirsten Kreikemeyer, Bernd Mathes, Stephanie Saxer, Sina
et. al:	No
DOI:	10.1098/rsos.171562 10.21256/zhaw-28950
Published in:	Royal Society Open Science
Volume(Issue):	5
Issue:	3
Page(s):	171562
Issue Date:	2018
Publisher / Ed. Institution:	The Royal Society Publishing
ISSN:	2054-5703
Language:	English
Subjects:	SAP hydrogel degradability; SAP hydrogel stiffness; Nanofibrillar architecture; Self-assembling peptides (SAP)
Subject (DDC):	660: Chemical engineering
Abstract:	Self-assembling peptide hydrogels can be modified regarding their biodegradability, their chemical and mechanical properties and their nanofibrillar structure. Thus, self-assembling peptide hydrogels might be suitable scaffolds for regenerative therapies and tissue engineering. Owing to the use of various peptide concentrations and buffer compositions, the self-assembling peptide hydrogels might be influenced regarding their mechanical characteristics. Therefore, the mechanical properties and stability of a set of self-assembling peptide hydrogels, consisting of 11 amino acids, made from four beta sheet self-assembling peptides in various peptide concentrations and buffer compositions were studied. The formed self-assembling peptide hydrogels exhibited stiffnesses ranging from 0.6 to 205 kPa. The hydrogel stiffness was mostly affected by peptide sequence followed by peptide concentration and buffer composition. All self-assembling peptide hydrogels examined provided a nanofibrillar network formation. A maximum self-assembling peptide hydrogel dissolution of 20% was observed for different buffer solutions after 7 days. The stability regarding enzymatic and bacterial digestion showed less degradation in comparison to the self-assembling peptide hydrogel dissolution rate in buffer. The tested set of self-assembling peptide hydrogels were able to form stable scaffolds and provided a broad spectrum of tissue-specific stiffnesses that are suitable for a regenerative therapy.
URI:	https://digitalcollection.zhaw.ch/handle/11475/28950
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Koch, F., Müller, M., König, F., Meyer, N., Gattlen, J., Pieles, U., Peters, K., Kreikemeyer, B., Mathes, S., & Saxer, S. (2018). Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition. Royal Society Open Science, 5(3), 171562. https://doi.org/10.1098/rsos.171562

Koch, F. et al. (2018) ‘Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition’, Royal Society Open Science, 5(3), p. 171562. Available at: https://doi.org/10.1098/rsos.171562.

F. Koch et al., “Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition,” Royal Society Open Science, vol. 5, no. 3, p. 171562, 2018, doi: 10.1098/rsos.171562.

KOCH, Franziska, Michael MÜLLER, Finja KÖNIG, Nina MEYER, Jasmin GATTLEN, Uwe PIELES, Kirsten PETERS, Bernd KREIKEMEYER, Stephanie MATHES und Sina SAXER, 2018. Mechanical characteristics of beta sheet-forming peptide hydrogels are dependent on peptide sequence, concentration and buffer composition. Royal Society Open Science. 2018. Bd. 5, Nr. 3, S. 171562. DOI 10.1098/rsos.171562

Koch, Franziska, Michael Müller, Finja König, Nina Meyer, Jasmin Gattlen, Uwe Pieles, Kirsten Peters, Bernd Kreikemeyer, Stephanie Mathes, and Sina Saxer. 2018. “Mechanical Characteristics of Beta Sheet-Forming Peptide Hydrogels Are Dependent on Peptide Sequence, Concentration and Buffer Composition.” Royal Society Open Science 5 (3): 171562. https://doi.org/10.1098/rsos.171562.

Koch, Franziska, et al. “Mechanical Characteristics of Beta Sheet-Forming Peptide Hydrogels Are Dependent on Peptide Sequence, Concentration and Buffer Composition.” Royal Society Open Science, vol. 5, no. 3, 2018, p. 171562, https://doi.org/10.1098/rsos.171562.