Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale

Blaschczok, Katharina; Löffelholz, Christian; Eibl-Schindler, Regine; Eibl, Dieter

doi:10.1002/elsc.201300069

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale
Authors:	Blaschczok, Katharina Löffelholz, Christian Eibl-Schindler, Regine Eibl, Dieter
DOI:	10.1002/elsc.201300069
Published in:	Engineering in Life Sciences
Volume(Issue):	14
Issue:	3
Page(s):	327
Pages to:	331
Issue Date:	May-2014
Publisher / Ed. Institution:	Wiley
ISSN:	1618-2863
Language:	English
Subjects:	Biphasic protein expression; Chinese hamster ovary cells; Hollow fiber module; SEAP (secreted alkaline phosphatase); Stirred single-use bioreactor
Subject (DDC):	660: Chemical engineering
Abstract:	The increasing implementation of single-use bioreactors arrived hand-in-hand with the development of new technologies contributing to increased productivity, process flexibility, and additional savings in time and costs. As a result, hollow fiber technology has recently gained renewed interest in upstream processing. Using a Chinese hamster ovary cell line in a biphasic protein production process with chemically defined minimal culture media, we combined Sartorius Stedim's BIOSTAT STR 50 L with GE Healthcare Life Sciences’ reusable Hollow Fiber Cartridge CFP-6-D-55A. After a 3-day feeding growth phase, secretion of the model protein secreted alkaline phosphatase (SEAP) was introduced by replacing the growth medium with production medium using cross-flow filtration. The process was then continued and harvested as a batch with temperature shift. High cell densities exceeding 1 × 107 cells mL−1 were achieved 5 days post inoculation and maximum secreted alkaline phosphatase activities of 24 U mL−1 11 days post inoculation. Our results showed that a further decrease in processing time is possible by reducing the number of diafiltration steps from three to two.
URI:	https://digitalcollection.zhaw.ch/handle/11475/2929
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Blaschczok, K., Löffelholz, C., Eibl-Schindler, R., & Eibl, D. (2014). Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences, 14(3), 327–331. https://doi.org/10.1002/elsc.201300069

Blaschczok, K. et al. (2014) ‘Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale’, Engineering in Life Sciences, 14(3), pp. 327–331. Available at: https://doi.org/10.1002/elsc.201300069.

K. Blaschczok, C. Löffelholz, R. Eibl-Schindler, and D. Eibl, “Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale,” Engineering in Life Sciences, vol. 14, no. 3, pp. 327–331, May 2014, doi: 10.1002/elsc.201300069.

BLASCHCZOK, Katharina, Christian LÖFFELHOLZ, Regine EIBL-SCHINDLER und Dieter EIBL, 2014. Combining single-use stirred bioreactor with standard cross-flow technology in biphasic protein production processes at pilot scale. Engineering in Life Sciences. Mai 2014. Bd. 14, Nr. 3, S. 327–331. DOI 10.1002/elsc.201300069

Blaschczok, Katharina, Christian Löffelholz, Regine Eibl-Schindler, and Dieter Eibl. 2014. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences 14 (3): 327–31. https://doi.org/10.1002/elsc.201300069.

Blaschczok, Katharina, et al. “Combining Single-Use Stirred Bioreactor with Standard Cross-Flow Technology in Biphasic Protein Production Processes at Pilot Scale.” Engineering in Life Sciences, vol. 14, no. 3, May 2014, pp. 327–31, https://doi.org/10.1002/elsc.201300069.