1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-29458
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEichenberger, Michael-
dc.contributor.authorSchwander, Thomas-
dc.contributor.authorHüppi, Sean-
dc.contributor.authorKreuzer, Jan-
dc.contributor.authorMittl, Peer R. E.-
dc.contributor.authorPeccati, Francesca-
dc.contributor.authorJiménez-Osés, Gonzalo-
dc.contributor.authorNaesby, Michael-
dc.contributor.authorBuller, Rebecca M.-
dc.date.accessioned2024-01-04T12:55:42Z-
dc.date.available2024-01-04T12:55:42Z-
dc.date.issued2023-
dc.identifier.issn2520-1158de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/29458-
dc.description.abstractAnthocyanins are ubiquitous plant pigments used in a variety of technological applications. Yet, after over a century of research, the penultimate biosynthetic step to anthocyanidins attributed to the action of leucoanthocyanidin dioxygenase has never been efficiently reconstituted outside plants, preventing the construction of heterologous cell factories. Through biochemical and structural analysis, here we show that anthocyanin-related glutathione transferases, currently implicated only in anthocyanin transport, catalyse an essential dehydration of the leucoanthocyanidin dioxygenase product, flavan-3,3,4-triol, to generate cyanidin. Building on this knowledge, introduction of anthocyanin-related glutathione transferases into a heterologous biosynthetic pathway in baker's yeast results in >35-fold increased anthocyanin production. In addition to unravelling the long-elusive anthocyanin biosynthesis, our findings pave the way for the colourants' heterologous microbial production and could impact the breeding of industrial and ornamental plants.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofNature Catalysisde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectBiocatalysisde_CH
dc.subjectMechanism of actionde_CH
dc.subjectSynthetic biologyde_CH
dc.subjectX-ray crystallographyde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleThe catalytic role of glutathione transferases in heterologous anthocyanin biosynthesisde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1038/s41929-023-01018-yde_CH
dc.identifier.doi10.21256/zhaw-29458-
dc.identifier.pmid37881531de_CH
zhaw.funding.euNode_CH
zhaw.issue10de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end938de_CH
zhaw.pages.start927de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume6de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf180544de_CH
zhaw.webfeedBiokatalysede_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
zhaw.relation.referenceshttps://doi.org/10.2210/pdb8agq/pdbde_CH
zhaw.relation.referenceshttps://doi.org/10.2210/pdb5f07/pdbde_CH
zhaw.relation.referenceshttps://doi.org/10.5281/zenodo.8069429de_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2023_Eichenberger-etal_Glutathione-transferases-in-heterologous-anthocyanin-biosynthesis.pdf12.42 MBAdobe PDFThumbnail
View/Open
Show simple item record
Eichenberger, M., Schwander, T., Hüppi, S., Kreuzer, J., Mittl, P. R. E., Peccati, F., Jiménez-Osés, G., Naesby, M., & Buller, R. M. (2023). The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis. Nature Catalysis, 6(10), 927–938. https://doi.org/10.1038/s41929-023-01018-y
Eichenberger, M. et al. (2023) ‘The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis’, Nature Catalysis, 6(10), pp. 927–938. Available at: https://doi.org/10.1038/s41929-023-01018-y.
M. Eichenberger et al., “The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis,” Nature Catalysis, vol. 6, no. 10, pp. 927–938, 2023, doi: 10.1038/s41929-023-01018-y.
EICHENBERGER, Michael, Thomas SCHWANDER, Sean HÜPPI, Jan KREUZER, Peer R. E. MITTL, Francesca PECCATI, Gonzalo JIMÉNEZ-OSÉS, Michael NAESBY und Rebecca M. BULLER, 2023. The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis. Nature Catalysis. 2023. Bd. 6, Nr. 10, S. 927–938. DOI 10.1038/s41929-023-01018-y
Eichenberger, Michael, Thomas Schwander, Sean Hüppi, Jan Kreuzer, Peer R. E. Mittl, Francesca Peccati, Gonzalo Jiménez-Osés, Michael Naesby, and Rebecca M. Buller. 2023. “The Catalytic Role of Glutathione Transferases in Heterologous Anthocyanin Biosynthesis.” Nature Catalysis 6 (10): 927–38. https://doi.org/10.1038/s41929-023-01018-y.
Eichenberger, Michael, et al. “The Catalytic Role of Glutathione Transferases in Heterologous Anthocyanin Biosynthesis.” Nature Catalysis, vol. 6, no. 10, 2023, pp. 927–38, https://doi.org/10.1038/s41929-023-01018-y.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.