Advances in noncanonical amino acid incorporation for enzyme engineering applications

Giger, Sandro; Buller, Rebecca

doi:10.2533/chimia.2023.395

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-29460

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Advances in noncanonical amino acid incorporation for enzyme engineering applications
Authors:	Giger, Sandro Buller, Rebecca
et. al:	No
DOI:	10.2533/chimia.2023.395 10.21256/zhaw-29460
Published in:	Chimia
Volume(Issue):	77
Issue:	6
Page(s):	395
Pages to:	402
Issue Date:	28-Jun-2023
Publisher / Ed. Institution:	Swiss Chemical Society
ISSN:	0009-4293 2673-2424
Language:	English
Subjects:	Chemical synthesis; Enzyme engineering; Enzyme mechanism; Genetic code expansion; New-to-nature enzyme; Noncanonical amino acid; Amino acid; Engineering
Subject (DDC):	660.6: Biotechnology
Abstract:	Incorporation of noncanonical amino acids (ncAAs) via genetic code expansion (GCE) opens up new possibilities for chemical biology. The technology has led to the development of novel xenobiotic enzymes with tailored properties which can serve as entry points into a multitude of applications, including protein conjugation, immobilization, or labeling. In this review, we discuss recent progress in the use of GCE to create biocatalysts possessing reaction repertoires that lie beyond what is achievable with canonical amino acids (cAAs). Furthermore, we highlight how GCE enables to gain mechanistic insights into protein function by the incorporation of judiciously selected ncAAs. As the amino acid alphabet continues to grow and improved tools for ncAA incorporation are being developed, we anticipate the creation of additional powerful biological catalysts for synthetic application which merge the chemical versatility of anthropogenic building blocks with the exquisite selectivities of enzymes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/29460
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Giger, S., & Buller, R. (2023). Advances in noncanonical amino acid incorporation for enzyme engineering applications. Chimia, 77(6), 395–402. https://doi.org/10.2533/chimia.2023.395

Giger, S. and Buller, R. (2023) ‘Advances in noncanonical amino acid incorporation for enzyme engineering applications’, Chimia, 77(6), pp. 395–402. Available at: https://doi.org/10.2533/chimia.2023.395.

S. Giger and R. Buller, “Advances in noncanonical amino acid incorporation for enzyme engineering applications,” Chimia, vol. 77, no. 6, pp. 395–402, Jun. 2023, doi: 10.2533/chimia.2023.395.

GIGER, Sandro und Rebecca BULLER, 2023. Advances in noncanonical amino acid incorporation for enzyme engineering applications. Chimia. 28 Juni 2023. Bd. 77, Nr. 6, S. 395–402. DOI 10.2533/chimia.2023.395

Giger, Sandro, and Rebecca Buller. 2023. “Advances in Noncanonical Amino Acid Incorporation for Enzyme Engineering Applications.” Chimia 77 (6): 395–402. https://doi.org/10.2533/chimia.2023.395.

Giger, Sandro, and Rebecca Buller. “Advances in Noncanonical Amino Acid Incorporation for Enzyme Engineering Applications.” Chimia, vol. 77, no. 6, June 2023, pp. 395–402, https://doi.org/10.2533/chimia.2023.395.