Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics

Seidel, Stefan; Maschke, Rüdiger W.; Mozaffari, Fruhar; Eibl-Schindler, Regine; Eibl, Dieter

Publication type:	Conference poster
Type of review:	Not specified
Title:	Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics
Authors:	Seidel, Stefan Maschke, Rüdiger W. Mozaffari, Fruhar Eibl-Schindler, Regine Eibl, Dieter
et. al:	No
Conference details:	Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel production routes and processes for bio-pharmaceuticals and industrial bioeconomy, Weimar, Germany, 15-17 May 2023
Issue Date:	15-May-2023
Language:	English
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Human embryonic kidney (HEK) cells are the second most used cell type for research and production of biopharmaceuticals after Chinese hamster ovary cells. In addition to vaccine production and the expression of viral vectors, various applications for chimeric antigen receptor T cell therapy are produced with these cells, too. For an optimised production, the bioprocess needs to be understood. One aspect is the engineering characterisation of the cultivation systems. In particular, the specific power input or the hydrodynamic stress are of interest and often used as scale-up criteria. The aim of this study was to determine the influence of hydrodynamic stress on maximum specific growth rate and viable cell density, as well as cell and aggregate size distribution when growing HEK 293 suspension cells. HEK Freestyle™ 293-F cells (Thermo Fisher Scientific) were grown in chemically defined medium (FreeStyle™ 293 from Gibco Thermo Fisher) in shake flasks with and without baffles and a Minifors 2 stirred bioreactor. The cultivations were performed in batch mode at specific power inputs between 63 Wm-3 and 451 Wm-3. Computational fluid dynamics (CFD) simulations were performed for all investigated cultivation systems to determine the hydrodynamic stress. Independently of the cultivation system and the type of mechanical power input, an aggregate size distribution was observed for all cultivations, which strictly follows a geometric distribution (Fig. 1). The only free parameter of the distribution corresponds to the fraction of non-aggregated cells and deviates only between 0.28 % and 5.95 % from the maximum likelihood estimate. With the help of the particle image velocity validated CFD simulations, it was shown that there is a linear relationship between the mean Kolmogorov length scale and the fraction of non-aggregated cells for the first time. This allows the prediction of the aggregate size distribution in batch cultivations using CFD simulations independent of the cultivation system.
URI:	https://digitalcollection.zhaw.ch/handle/11475/29571
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Seidel, S., Maschke, R. W., Mozaffari, F., Eibl-Schindler, R., & Eibl, D. (2023, May 15). Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics. Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel Production Routes and Processes for Bio-Pharmaceuticals and Industrial Bioeconomy, Weimar, Germany, 15-17 May 2023.

Seidel, S. et al. (2023) ‘Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics’, in Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel production routes and processes for bio-pharmaceuticals and industrial bioeconomy, Weimar, Germany, 15-17 May 2023.

S. Seidel, R. W. Maschke, F. Mozaffari, R. Eibl-Schindler, and D. Eibl, “Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics,” in Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel production routes and processes for bio-pharmaceuticals and industrial bioeconomy, Weimar, Germany, 15-17 May 2023, May 2023.

SEIDEL, Stefan, Rüdiger W. MASCHKE, Fruhar MOZAFFARI, Regine EIBL-SCHINDLER und Dieter EIBL, 2023. Prediction of the aggregate size distribution of HEK293 cells based on computational fluid dynamics. In: Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel production routes and processes for bio-pharmaceuticals and industrial bioeconomy, Weimar, Germany, 15-17 May 2023. Conference poster. 15 Mai 2023

Seidel, Stefan, Rüdiger W. Maschke, Fruhar Mozaffari, Regine Eibl-Schindler, and Dieter Eibl. 2023. “Prediction of the Aggregate Size Distribution of HEK293 Cells Based on Computational Fluid Dynamics.” Conference poster. In Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel Production Routes and Processes for Bio-Pharmaceuticals and Industrial Bioeconomy, Weimar, Germany, 15-17 May 2023.

Seidel, Stefan, et al. “Prediction of the Aggregate Size Distribution of HEK293 Cells Based on Computational Fluid Dynamics.” Himmelfahrtstagung on Bioprocess Engineering 2023 - Novel Production Routes and Processes for Bio-Pharmaceuticals and Industrial Bioeconomy, Weimar, Germany, 15-17 May 2023, 2023.