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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-29847
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dc.contributor.authorRaschmanová, Hana-
dc.contributor.authorWeninger, Astrid-
dc.contributor.authorKnejzlík, Zdeněk-
dc.contributor.authorMelzoch, Karel-
dc.contributor.authorKovar, Karin-
dc.date.accessioned2024-02-08T14:29:48Z-
dc.date.available2024-02-08T14:29:48Z-
dc.date.issued2021-05-26-
dc.identifier.issn0175-7598de_CH
dc.identifier.issn1432-0614de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/29847-
dc.descriptionErworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch)de_CH
dc.description.abstractFolding and processing of proteins in the endoplasmic reticulum (ER) are major impediments in the production and secretion of proteins from Pichia pastoris (Komagataella sp.). Overexpression of recombinant genes can overwhelm the innate secretory machinery of the P. pastoris cell, and incorrectly folded proteins may accumulate inside the ER. To restore proper protein folding, the cell naturally triggers an unfolded protein response (UPR) pathway, which upregulates the expression of genes coding for chaperones and other folding-assisting proteins (e.g., Kar2p, Pdi1, Ero1p) via the transcription activator Hac1p. Unfolded/misfolded proteins that cannot be repaired are degraded via the ER-associated degradation (ERAD) pathway, which decreases productivity. Co-expression of selected UPR genes, along with the recombinant gene of interest, is a common approach to enhance the production of properly folded, secreted proteins. Such an approach, however, is not always successful and sometimes, protein productivity decreases because of an unbalanced UPR. This review summarizes successful chaperone co-expression strategies in P. pastoris that are specifically related to overproduction of foreign proteins and the UPR. In addition, it illustrates possible negative effects on the cell's physiology and productivity resulting from genetic engineering of the UPR pathway. We have focused on Pichia's potential for commercial production of valuable proteins and we aim to optimize molecular designs so that production strains can be tailored to suit a specific heterologous product.de_CH
dc.language.isoende_CH
dc.publisherSpringerde_CH
dc.relation.ispartofApplied Microbiology and Biotechnologyde_CH
dc.rightshttps://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectChaperonede_CH
dc.subjectCo-expression strategyde_CH
dc.subjectFolding and secretionde_CH
dc.subjectPichia pastorisde_CH
dc.subjectProductivity of recombinant protein productionde_CH
dc.subjectUnfolded protein response (UPR)de_CH
dc.subjectRecombinant proteinde_CH
dc.subjectSaccharomycetalesde_CH
dc.subjectFungal proteinde_CH
dc.subject.ddc579: Mikrobiologiede_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleEngineering of the unfolded protein response pathway in Pichia pastoris : enhancing production of secreted recombinant proteinsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1007/s00253-021-11336-5de_CH
dc.identifier.doi10.21256/zhaw-29847-
dc.identifier.pmid34037840de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end4414de_CH
zhaw.pages.start4397de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume105de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Raschmanová, H., Weninger, A., Knejzlík, Z., Melzoch, K., & Kovar, K. (2021). Engineering of the unfolded protein response pathway in Pichia pastoris : enhancing production of secreted recombinant proteins. Applied Microbiology and Biotechnology, 105, 4397–4414. https://doi.org/10.1007/s00253-021-11336-5
Raschmanová, H. et al. (2021) ‘Engineering of the unfolded protein response pathway in Pichia pastoris : enhancing production of secreted recombinant proteins’, Applied Microbiology and Biotechnology, 105, pp. 4397–4414. Available at: https://doi.org/10.1007/s00253-021-11336-5.
H. Raschmanová, A. Weninger, Z. Knejzlík, K. Melzoch, and K. Kovar, “Engineering of the unfolded protein response pathway in Pichia pastoris : enhancing production of secreted recombinant proteins,” Applied Microbiology and Biotechnology, vol. 105, pp. 4397–4414, May 2021, doi: 10.1007/s00253-021-11336-5.
RASCHMANOVÁ, Hana, Astrid WENINGER, Zdeněk KNEJZLÍK, Karel MELZOCH und Karin KOVAR, 2021. Engineering of the unfolded protein response pathway in Pichia pastoris : enhancing production of secreted recombinant proteins. Applied Microbiology and Biotechnology. 26 Mai 2021. Bd. 105, S. 4397–4414. DOI 10.1007/s00253-021-11336-5
Raschmanová, Hana, Astrid Weninger, Zdeněk Knejzlík, Karel Melzoch, and Karin Kovar. 2021. “Engineering of the Unfolded Protein Response Pathway in Pichia Pastoris : Enhancing Production of Secreted Recombinant Proteins.” Applied Microbiology and Biotechnology 105 (May): 4397–4414. https://doi.org/10.1007/s00253-021-11336-5.
Raschmanová, Hana, et al. “Engineering of the Unfolded Protein Response Pathway in Pichia Pastoris : Enhancing Production of Secreted Recombinant Proteins.” Applied Microbiology and Biotechnology, vol. 105, May 2021, pp. 4397–414, https://doi.org/10.1007/s00253-021-11336-5.


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