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dc.contributor.authorTrifan, Adriana-
dc.contributor.authorCzerwińska, Monika E-
dc.contributor.authorZengin, Gokhan-
dc.contributor.authorEsslinger, Nils-
dc.contributor.authorGrubelnik, Andreas-
dc.contributor.authorWolfram, Evelyn-
dc.contributor.authorSkalicka-Woźniak, Krystyna-
dc.contributor.authorLuca, Simon Vlad-
dc.date.accessioned2024-02-29T13:12:24Z-
dc.date.available2024-02-29T13:12:24Z-
dc.date.issued2023-03-01-
dc.identifier.issn0378-8741de_CH
dc.identifier.issn1872-7573de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/30017-
dc.description.abstractEthnopharmacological relevance: Comfrey (Symphytum officinale L., Boraginaceae) root preparations are used as both traditional remedies and therapeutic agents in treating pain and inflammation associated with joint, bone, and muscle ailments. Even though numerous phytochemicals contribute to the beneficial effects of comfrey, the presence of toxic pyrrolizidine alkaloids (PAs) overshadows its uses. Aim of the study: In this work, different PA-/mucilage-depleted/undepleted comfrey root extracts were subjected to detailed phytochemical characterization and biological evaluation. Materials and methods: The phytochemical profiling was performed by LC-HRMS/MS. The quantification of PAs and major phenolic compounds was carried out by LC-MS/MS and LC-DAD. Antioxidant and enzyme inhibitory activity was determined by in vitro free radical scavenging, ion reducing, metal chelating, cholinesterase, tyrosinase, amylase, and glucosidase assays. Using an ex vivo model of LPS-stimulated neutrophils, their viability (as measured by flow cytometry) and the release of IL-1β, IL-8, and TNF-α were determined (ELISA assay). Results: 12 phenolic acids, six PAs, three organic acids, two fatty acids, and two sugars were identified in the obtained comfrey extracts. The PA-depleted materials contained PAs levels below 2 ppm, whereas the removal of mucilage increased the content of rosmarinic acid, globoidnan A, globoidnan B, and rabdosiin. PA-depletion did not significantly affect the antioxidant potential. However, the radical scavenging and metal reducing properties were higher in the mucilage-depleted extracts. Neither PA-depletion nor mucilage-depletion had considerable effects on the in vitro inhibitory activity of cholinesterases, tyrosinase, amylase, and glucosidase or release of ex vivo pro-inflammatory cytokines (e.g., IL-1β, IL-8, and TNF-α) in LPS-stimulated neutrophils. Conclusions: In light of their superior safety profiles, PA-depleted comfrey extracts can be utilized further in cosmetic and pharmaceutical products.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofJournal of Ethnopharmacologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectDepletionde_CH
dc.subjectGloboidnande_CH
dc.subjectPro-inflammatory cytokinesde_CH
dc.subjectPyrrolizidine alkaloidsde_CH
dc.subjectSymphytum officinalede_CH
dc.subjectChromatography, Liquidde_CH
dc.subjectAntioxidantsde_CH
dc.subjectMonophenol monooxygenasede_CH
dc.subjectInterleukin-8de_CH
dc.subjectLipopolysaccharidesde_CH
dc.subjectTumor necrosis factor-alphade_CH
dc.subjectTandem mass spectrometryde_CH
dc.subjectPlant extractsde_CH
dc.subjectPhytochemicalsde_CH
dc.subjectComfreyde_CH
dc.subjectPyrrolizidine alkaloidsde_CH
dc.subjectBoraginaceaede_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.titleInfluence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extractsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.jep.2022.116010de_CH
dc.identifier.pmid36493995de_CH
zhaw.funding.euNode_CH
zhaw.issue116010de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume303de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedPhytopharmaziede_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
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Trifan, A., Czerwińska, M. E., Zengin, G., Esslinger, N., Grubelnik, A., Wolfram, E., Skalicka-Woźniak, K., & Luca, S. V. (2023). Influence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extracts. Journal of Ethnopharmacology, 303(116010). https://doi.org/10.1016/j.jep.2022.116010
Trifan, A. et al. (2023) ‘Influence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extracts’, Journal of Ethnopharmacology, 303(116010). Available at: https://doi.org/10.1016/j.jep.2022.116010.
A. Trifan et al., “Influence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extracts,” Journal of Ethnopharmacology, vol. 303, no. 116010, Mar. 2023, doi: 10.1016/j.jep.2022.116010.
TRIFAN, Adriana, Monika E CZERWIŃSKA, Gokhan ZENGIN, Nils ESSLINGER, Andreas GRUBELNIK, Evelyn WOLFRAM, Krystyna SKALICKA-WOŹNIAK und Simon Vlad LUCA, 2023. Influence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extracts. Journal of Ethnopharmacology. 1 März 2023. Bd. 303, Nr. 116010. DOI 10.1016/j.jep.2022.116010
Trifan, Adriana, Monika E Czerwińska, Gokhan Zengin, Nils Esslinger, Andreas Grubelnik, Evelyn Wolfram, Krystyna Skalicka-Woźniak, and Simon Vlad Luca. 2023. “Influence of Pyrrolizidine Alkaloids Depletion upon the Biological Activity of Symphytum Officinale L. Extracts.” Journal of Ethnopharmacology 303 (116010). https://doi.org/10.1016/j.jep.2022.116010.
Trifan, Adriana, et al. “Influence of Pyrrolizidine Alkaloids Depletion upon the Biological Activity of Symphytum Officinale L. Extracts.” Journal of Ethnopharmacology, vol. 303, no. 116010, Mar. 2023, https://doi.org/10.1016/j.jep.2022.116010.


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