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dc.contributor.authorTeale, Misha-
dc.contributor.authorJossen, Valentin-
dc.contributor.authorEibl, Dieter-
dc.contributor.authorEibl-Schindler, Regine-
dc.date.accessioned2024-03-16T10:36:11Z-
dc.date.available2024-03-16T10:36:11Z-
dc.date.issued2021-10-06-
dc.identifier.isbn978-1-0716-2017-5de_CH
dc.identifier.isbn978-1-0716-2018-2de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/30295-
dc.description.abstractIn recent years, the use of hMSCs, which may be isolated from adipose tissue among others, for the treatment of diseases has increased significantly. The cell quantities required for such therapeutic approaches, between 1012 and 1013, have thus far been predominantly produced using commercially available multi-tray systems, such as the Cell Factory (Thermo Fisher Scientific) or HYPERStack (Corning), which can be purchased with up to 40 layers. However, the handling of these planar multilayer systems is difficult, and process monitoring opportunities remain limited. Here, automated stirred single-use bioreactors provide a viable alternative to the time-consuming multiplication of cells using such planar systems, while still managing to achieve the desired clinically relevant quantities. In these stirred single-use systems, adherent cells are predominantly cultivated in suspension up to pilot scale using carrier materials, also referred to as microcarriers (MCs).This chapter describes the steps which need to be realized to guarantee successful hMSC expansion within a stirred single-use bioreactor (Eppendorf's BioBLU® 0.3c) operated using MCs under serum- and xeno-free conditions at benchtop scale. The cultivations were performed using an immortalized human adipose-derived mesenchymal stem cell (hASC) line, hence referred to as hASC52telo, and a new chemically defined, xeno-free medium, hence referred to as the UrSuppe formulation. Spinner flask cultivations were performed under comparable process conditions.de_CH
dc.language.isoende_CH
dc.publisherHumanade_CH
dc.relation.ispartofBioreactors in Stem Cell Biology : Methods and Protocolsde_CH
dc.relation.ispartofseriesMethods in Molecular Biologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBioBLU® 0.3c bioreactorde_CH
dc.subjectCell expansionde_CH
dc.subjectMicrocarrierde_CH
dc.subjectUrSuppede_CH
dc.subjecthTERT immortalized ASC5Telo cellde_CH
dc.subjectSingle-usede_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleChemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactorde_CH
dc.typeBuchbeitragde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.publisher.placeNew York, NYde_CH
dc.identifier.doi10.1007/7651_2021_426de_CH
dc.identifier.pmid34611815de_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end111de_CH
zhaw.pages.start83de_CH
zhaw.parentwork.editorTurksen, Kursad-
zhaw.publication.statuspublishedVersionde_CH
zhaw.series.number2436de_CH
zhaw.publication.reviewEditorial reviewde_CH
zhaw.webfeed3D Gewebe und Biofabrikationde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Teale, M., Jossen, V., Eibl, D., & Eibl-Schindler, R. (2021). Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor. In K. Turksen (Ed.), Bioreactors in Stem Cell Biology : Methods and Protocols (pp. 83–111). Humana. https://doi.org/10.1007/7651_2021_426
Teale, M. et al. (2021) ‘Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor’, in K. Turksen (ed.) Bioreactors in Stem Cell Biology : Methods and Protocols. New York, NY: Humana, pp. 83–111. Available at: https://doi.org/10.1007/7651_2021_426.
M. Teale, V. Jossen, D. Eibl, and R. Eibl-Schindler, “Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor,” in Bioreactors in Stem Cell Biology : Methods and Protocols, K. Turksen, Ed. New York, NY: Humana, 2021, pp. 83–111. doi: 10.1007/7651_2021_426.
TEALE, Misha, Valentin JOSSEN, Dieter EIBL und Regine EIBL-SCHINDLER, 2021. Chemically defined, xeno-free expansion of human mesenchymal stem cells (hMSCs) on benchtop-scale using a stirred single-use bioreactor. In: Kursad TURKSEN (Hrsg.), Bioreactors in Stem Cell Biology : Methods and Protocols. New York, NY: Humana. S. 83–111. ISBN 978-1-0716-2017-5
Teale, Misha, Valentin Jossen, Dieter Eibl, and Regine Eibl-Schindler. 2021. “Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor.” In Bioreactors in Stem Cell Biology : Methods and Protocols, edited by Kursad Turksen, 83–111. New York, NY: Humana. https://doi.org/10.1007/7651_2021_426.
Teale, Misha, et al. “Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor.” Bioreactors in Stem Cell Biology : Methods and Protocols, edited by Kursad Turksen, Humana, 2021, pp. 83–111, https://doi.org/10.1007/7651_2021_426.


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