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https://doi.org/10.21256/zhaw-30490| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors |
| Authors: | Ott, Vivian Ott, Jan Eibl, Dieter Eibl-Schindler, Regine |
| et. al: | No |
| DOI: | 10.3390/pr12040806 10.21256/zhaw-30490 |
| Published in: | Processes |
| Volume(Issue): | 12 |
| Issue: | 4 |
| Page(s): | 806 |
| Issue Date: | 17-Apr-2024 |
| Publisher / Ed. Institution: | MDPI |
| ISSN: | 2227-9717 |
| Language: | English |
| Subjects: | Automation; Chinese hamster ovary cells; Monoclonal antibodies; Upstream process; Process intensification; Perfusion |
| Subject (DDC): | 660: Chemical engineering |
| Abstract: | Modern production processes for biopharmaceuticals often work with very high cell densities. Moreover, there is a trend towards moving from fed-batch to continuous perfusion processes; a development that is influencing the requirements for bioreactor design and process control. In this study, the transfer of fed-batch and perfusion experiments between different cylindrical stirred lab-scale bioreactors and Thermo Scientific’sTM (Waltham, MA, USA) cubical HyPerformaTM DynaDriveTM Single-Use Bioreactor was investigated. Different scaling parameters were used, which were selected based on the requirements of the respective processes. Peak cell densities of up to 49 × 106 cells mL−1 and antibody titers of up to 5.2 g L−1 were achieved in 15- to 16-day fed-batch experiments. In 50-day perfusion cultivations, a viable cell volume of >100 mm3 mL−1 was maintained and more than 1 g L−1 d−1 of antibodies were harvested. The perfusion processes were automated with both cell bleed control and glucose concentration control. Cell retention was performed using Repligen’s (Waltham, MA, USA) XCell® ATF perfusion systems and single-use devices. In summary, approaches for successfully scaling highly productive fed-batch and perfusion processes between geometrically dissimilar lab and pilot scale bioreactors were demonstrated. The advantages of perfusion in comparison to fed-batch processes were also observed. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/30490 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2024_Ott-etal_Scaling-antibody-production-bioreactors_MDPI.pdf | 2.08 MB | Adobe PDF |  View/Open |
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Ott, V., Ott, J., Eibl, D., & Eibl-Schindler, R. (2024). Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors. Processes, 12(4), 806. https://doi.org/10.3390/pr12040806
Ott, V. et al. (2024) ‘Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors’, Processes, 12(4), p. 806. Available at: https://doi.org/10.3390/pr12040806.
V. Ott, J. Ott, D. Eibl, and R. Eibl-Schindler, “Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors,” Processes, vol. 12, no. 4, p. 806, Apr. 2024, doi: 10.3390/pr12040806.
OTT, Vivian, Jan OTT, Dieter EIBL und Regine EIBL-SCHINDLER, 2024. Scaling fed-batch and perfusion antibody production processes in geometrically dissimilar stirred bioreactors. Processes. 17 April 2024. Bd. 12, Nr. 4, S. 806. DOI 10.3390/pr12040806
Ott, Vivian, Jan Ott, Dieter Eibl, and Regine Eibl-Schindler. 2024. “Scaling Fed-Batch and Perfusion Antibody Production Processes in Geometrically Dissimilar Stirred Bioreactors.” Processes 12 (4): 806. https://doi.org/10.3390/pr12040806.
Ott, Vivian, et al. “Scaling Fed-Batch and Perfusion Antibody Production Processes in Geometrically Dissimilar Stirred Bioreactors.” Processes, vol. 12, no. 4, Apr. 2024, p. 806, https://doi.org/10.3390/pr12040806.
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