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dc.contributor.authorBroggini, Giovanni A. L.-
dc.contributor.authorWöhner, Thomas-
dc.contributor.authorFahrentrapp, Johannes-
dc.contributor.authorKost, Thomas D.-
dc.contributor.authorFlachowsky, Henryk-
dc.contributor.authorPeil, Andreas-
dc.contributor.authorHanke, Maria-Viola-
dc.contributor.authorRichter, Klaus-
dc.contributor.authorPatocchi, Andrea-
dc.contributor.authorGessler, Cesare-
dc.date.accessioned2018-03-20T14:41:12Z-
dc.date.available2018-03-20T14:41:12Z-
dc.date.issued2014-03-
dc.identifier.issn1467-7644de_CH
dc.identifier.issn1467-7652de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/4095-
dc.description.abstractThe fire blight susceptible apple cultivar Malus × domestica Borkh. cv. ‘Gala’ was transformed with the candidate fire blight resistance gene FB_MR5 originating from the crab apple accession Malus × robusta 5 (Mr5). A total of five different transgenic lines were obtained. All transgenic lines were shown to be stably transformed and originate from different transgenic events. The transgenic lines express the FB_MR5 either driven by the constitutive CaMV 35S promoter and the ocs terminator or by its native promoter and terminator sequences. Phenotyping experiments were performed with Mr5-virulent and Mr5-avirulent strains of Erwinia amylovora, the causal agent of fire blight. Significantly less disease symptoms were detected on transgenic lines after inoculation with two different Mr5-avirulent E. amylovora strains, while significantly more shoot necrosis was observed after inoculation with the Mr5-virulent mutant strain ZYRKD3_1. The results of these experiments demonstrated the ability of a single gene isolated from the native gene pool of apple to protect a susceptible cultivar from fire blight. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host–pathogen relationship Mr5–E. amylovora.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofPlant Biotechnology Journalde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectGenetic engineeringde_CH
dc.subjectGMOde_CH
dc.subjectResistancede_CH
dc.subjectWeinbaude_CH
dc.subject.ddc634: Obstanlagen, Früchte und Forstwirtschaftde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleEngineering fire blight resistance into the apple cultivar 'Gala' using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovannide_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1111/pbi.12177de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end733de_CH
zhaw.pages.start728de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume12de_CH
zhaw.publication.reviewNot specifiedde_CH
zhaw.webfeedHortikulturde_CH
zhaw.funding.zhawOccurence of grape phylloxera (Dactulosphaira vitifoliae) in Switzerlandde_CH
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Broggini, G. A. L., Wöhner, T., Fahrentrapp, J., Kost, T. D., Flachowsky, H., Peil, A., Hanke, M.-V., Richter, K., Patocchi, A., & Gessler, C. (2014). Engineering fire blight resistance into the apple cultivar ‘Gala’ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni. Plant Biotechnology Journal, 12(6), 728–733. https://doi.org/10.1111/pbi.12177
Broggini, G.A.L. et al. (2014) ‘Engineering fire blight resistance into the apple cultivar “Gala” using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni’, Plant Biotechnology Journal, 12(6), pp. 728–733. Available at: https://doi.org/10.1111/pbi.12177.
G. A. L. Broggini et al., “Engineering fire blight resistance into the apple cultivar ‘Gala’ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni,” Plant Biotechnology Journal, vol. 12, no. 6, pp. 728–733, Mar. 2014, doi: 10.1111/pbi.12177.
BROGGINI, Giovanni A. L., Thomas WÖHNER, Johannes FAHRENTRAPP, Thomas D. KOST, Henryk FLACHOWSKY, Andreas PEIL, Maria-Viola HANKE, Klaus RICHTER, Andrea PATOCCHI und Cesare GESSLER, 2014. Engineering fire blight resistance into the apple cultivar ‚Gala‘ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni. Plant Biotechnology Journal. März 2014. Bd. 12, Nr. 6, S. 728–733. DOI 10.1111/pbi.12177
Broggini, Giovanni A. L., Thomas Wöhner, Johannes Fahrentrapp, Thomas D. Kost, Henryk Flachowsky, Andreas Peil, Maria-Viola Hanke, Klaus Richter, Andrea Patocchi, and Cesare Gessler. 2014. “Engineering Fire Blight Resistance into the Apple Cultivar ‘Gala’ Using the FB_MR5 CC-NBS-LRR Resistance Gene of Malus × Robusta 5 Giovanni.” Plant Biotechnology Journal 12 (6): 728–33. https://doi.org/10.1111/pbi.12177.
Broggini, Giovanni A. L., et al. “Engineering Fire Blight Resistance into the Apple Cultivar ‘Gala’ Using the FB_MR5 CC-NBS-LRR Resistance Gene of Malus × Robusta 5 Giovanni.” Plant Biotechnology Journal, vol. 12, no. 6, Mar. 2014, pp. 728–33, https://doi.org/10.1111/pbi.12177.


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