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https://doi.org/10.21256/zhaw-2679Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Fahrentrapp, Johannes | - |
| dc.contributor.author | Michl, Gertraud | - |
| dc.contributor.author | Breuer, Michael | - |
| dc.date.accessioned | 2018-03-20T14:43:50Z | - |
| dc.date.available | 2018-03-20T14:43:50Z | - |
| dc.date.issued | 2013 | - |
| dc.identifier.issn | 0042-7500 | de_CH |
| dc.identifier.issn | 2367-4156 | de_CH |
| dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/4096 | - |
| dc.description.abstract | In Europe's vineyards "Bois noir" (BN) is an expanding yellows disease on Vitis vinifera. It is associated with phytoplasmas of the stolbur group (16SrXII-A). Two subtypes are important, one is associated with Urtica dioica and one with Convolvulus arvensis. Both phytoplasma types are transmitted by the insect Hyalesthes obsoletus. A nucleic acid extraction method for V. vinifera and H. obsoletus was developed together with a real time PCR (qPCR) assay based on a polymorphic sequence with homology to a putative dimethyladenosine transferase. The comparison of the conventional detection method with the qPCR assay of 40 insect and 40 V. vinifera samples showed a 10% higher sensitivity of qPCR in plant samples. The titer of phytoplasmas in H. obsoletus was 2643-fold increased in the strongest infected samples compared to the lowest ones. The results suggest this real-time PCR as a valid and fast alternative procedure for the detection and quantification of BN phytoplasmas. The assay allows to discriminate the two phytoplasma types and to quantify phytoplasmas in H. obsoletus. | de_CH |
| dc.language.iso | en | de_CH |
| dc.publisher | Julius Kühn Institute for Grapevine Breeding | de_CH |
| dc.relation.ispartof | Vitis - Journal of Grapevine Research | de_CH |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | de_CH |
| dc.subject | Phytoplasma disease | de_CH |
| dc.subject | Quantitative real-time PCR (qPCR) | de_CH |
| dc.subject | "Vergilbungskrankheit" | de_CH |
| dc.subject | Grape disease | de_CH |
| dc.subject.ddc | 572: Biochemie | de_CH |
| dc.subject.ddc | 634: Obstanlagen, Früchte und Forstwirtschaft | de_CH |
| dc.title | Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue | de_CH |
| dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
| dcterms.type | Text | de_CH |
| zhaw.departement | Life Sciences und Facility Management | de_CH |
| zhaw.organisationalunit | Institut für Umwelt und Natürliche Ressourcen (IUNR) | de_CH |
| dc.identifier.doi | 10.21256/zhaw-2679 | - |
| zhaw.funding.eu | No | de_CH |
| zhaw.issue | 2 | de_CH |
| zhaw.originated.zhaw | Yes | de_CH |
| zhaw.pages.end | 89 | de_CH |
| zhaw.pages.start | 85 | de_CH |
| zhaw.publication.status | publishedVersion | de_CH |
| zhaw.volume | 52 | de_CH |
| zhaw.publication.review | Peer review (Publikation) | de_CH |
| zhaw.webfeed | Hortikultur | de_CH |
| zhaw.funding.zhaw | Detection of Bois noir phytoplasma in the vectoring ciciid Hyalethes obsoletus. | de_CH |
| Appears in collections: | Publikationen Life Sciences und Facility Management | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2013_Fahrentrapp_Quantitative PCR assay_VITIS.pdf | 525.04 kB | Adobe PDF |  View/Open |
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Fahrentrapp, J., Michl, G., & Breuer, M. (2013). Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue. Vitis - Journal of Grapevine Research, 52(2), 85–89. https://doi.org/10.21256/zhaw-2679
Fahrentrapp, J., Michl, G. and Breuer, M. (2013) ‘Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue’, Vitis - Journal of Grapevine Research, 52(2), pp. 85–89. Available at: https://doi.org/10.21256/zhaw-2679.
J. Fahrentrapp, G. Michl, and M. Breuer, “Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue,” Vitis - Journal of Grapevine Research, vol. 52, no. 2, pp. 85–89, 2013, doi: 10.21256/zhaw-2679.
FAHRENTRAPP, Johannes, Gertraud MICHL und Michael BREUER, 2013. Quantitative PCR assay for detection of bois noir phytoplasmas in grape and insect tissue. Vitis - Journal of Grapevine Research. 2013. Bd. 52, Nr. 2, S. 85–89. DOI 10.21256/zhaw-2679
Fahrentrapp, Johannes, Gertraud Michl, and Michael Breuer. 2013. “Quantitative PCR Assay for Detection of Bois Noir Phytoplasmas in Grape and Insect Tissue.” Vitis - Journal of Grapevine Research 52 (2): 85–89. https://doi.org/10.21256/zhaw-2679.
Fahrentrapp, Johannes, et al. “Quantitative PCR Assay for Detection of Bois Noir Phytoplasmas in Grape and Insect Tissue.” Vitis - Journal of Grapevine Research, vol. 52, no. 2, 2013, pp. 85–89, https://doi.org/10.21256/zhaw-2679.
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