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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: A candidate gene for fire blight resistance in Malus × robusta 5 is coding for a CC-NBS-LRR
Authors: Fahrentrapp, Johannes
Broggini, Giovanni A. L.
Kellerhals, Markus
Peil, Andreas
Richter, Klaus
Zini, Elena
Gessler, Cesare
DOI: 10.21256/zhaw-1825
Published in: Tree Genetics & Genomes
Volume(Issue): 9
Issue: 1
Page(s): 237
Pages to: 251
Issue Date: Feb-2013
Publisher / Ed. Institution: Springer
ISSN: 1614-2942
Language: English
Subjects: Resistance gene; Apple; Weinbau; Fire blight
Subject (DDC): 634: Orchards, fruits and forestry
Abstract: Fire blight is the most important bacterial disease in apple (Malus ×  domestica) and pear (Pyrus communis) production. Today, the causal bacterium Erwinia amylovora is present in many apple- and pear-growing areas. We investigated the natural resistance of the wild apple Malus ×  robusta 5 against E. amylovora, previously mapped to linkage group 3. With a fine-mapping approach on a population of 2,133 individuals followed by phenotyping of the recombinants from the region of interest, we developed flanking markers useful for marker-assisted selection. Open reading frames were predicted on the sequence of a BAC spanning the resistance locus. One open reading frame coded for a protein belonging to the NBS–LRR family. The in silico investigation of the structure of the candidate resistance gene against fire blight of M. ×  robusta 5, FB_MR5, led us hypothesize the presence of a coiled-coil region followed by an NBS and an LRR-like structure with the consensus ‘LxxLx[IL]xxCxxLxxL’. The function of FB_MR5 was predicted in agreement with the decoy/guard model, that FB_MR5 monitors the transcribed RIN4_MR5, a homolog of RIN4 of Arabidopsis thaliana that could interact with the previously described effector AvrRpt2EA of E. amylovora.
Further description: Erworben im Rahmen der Schweizer Nationallizenzen (
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Appears in collections:Publikationen Life Sciences und Facility Management

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