| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes |
| Authors: | Stöckli, Jacqueline Rohrer, Jack |
| DOI: | 10.1091/mbc.e03-11-0808 |
| Published in: | Molecular Biology of the Cell |
| Volume(Issue): | 15 |
| Issue: | 6 |
| Page(s): | 2617 |
| Pages to: | 2626 |
| Issue Date: | 2004 |
| Publisher / Ed. Institution: | American Society for Cell Biology |
| ISSN: | 1939-4586 1059-1524 |
| Language: | English |
| Subject (DDC): | 571: Physiology and related subjects 572: Biochemistry |
| Abstract: | The cation-dependent mannose 6-phosphate receptor (CD-MPR) mediates the transport of lysosomal enzymes from the trans-Golgi network to endosomes. Evasion of lysosomal degradation of the CD-MPR requires reversible palmitoylation of a cysteine residue in its cytoplasmic tail. Because palmitoylation is reversible and essential for correct trafficking, it presents a potential regulatory mechanism for the sorting signals within the cytoplasmic domain of the CD-MPR. Characterization of the palmitoylation performing an in vitro palmitoylation assay by using purified full-length CD-MPR revealed that palmitoylation of the CD-MPR occurs enzymatically by a membrane-bound palmitoyltransferase. In addition, analysis of the localization revealed that the palmitoyltransferase cycles between endosomes and the plasma membrane. This was identified by testing fractions from HeLa cell homogenate separated on a density gradient in the in vitro palmitoylation assay and further confirmed by in vivo labeling experiments by using different treatments to block specific protein trafficking steps within the cell. We identified a novel palmitoyltransferase activity in the endocytic pathway responsible for palmitoylation of the CD-MPR. The localization of the palmitoyltransferase not only fulfills the requirement of our hypothesis to be a regulator of the intracellular trafficking of the CD-MPR but also may affect the sorting/activity of other receptors cycling through endosomes. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6794 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Stöckli, J., & Rohrer, J. (2004). The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes. Molecular Biology of the Cell, 15(6), 2617–2626. https://doi.org/10.1091/mbc.e03-11-0808
Stöckli, J. and Rohrer, J. (2004) ‘The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes’, Molecular Biology of the Cell, 15(6), pp. 2617–2626. Available at: https://doi.org/10.1091/mbc.e03-11-0808.
J. Stöckli and J. Rohrer, “The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes,” Molecular Biology of the Cell, vol. 15, no. 6, pp. 2617–2626, 2004, doi: 10.1091/mbc.e03-11-0808.
STÖCKLI, Jacqueline und Jack ROHRER, 2004. The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes. Molecular Biology of the Cell. 2004. Bd. 15, Nr. 6, S. 2617–2626. DOI 10.1091/mbc.e03-11-0808
Stöckli, Jacqueline, and Jack Rohrer. 2004. “The Palmitoyltransferase of the Cation-Dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes.” Molecular Biology of the Cell 15 (6): 2617–26. https://doi.org/10.1091/mbc.e03-11-0808.
Stöckli, Jacqueline, and Jack Rohrer. “The Palmitoyltransferase of the Cation-Dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes.” Molecular Biology of the Cell, vol. 15, no. 6, 2004, pp. 2617–26, https://doi.org/10.1091/mbc.e03-11-0808.
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