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dc.contributor.authorSchaub, Beat E-
dc.contributor.authorNair, Prashant-
dc.contributor.authorRohrer, Jack-
dc.date.accessioned2018-06-13T08:30:15Z-
dc.date.available2018-06-13T08:30:15Z-
dc.date.issued2005-07-01-
dc.identifier.issn1934-2500de_CH
dc.identifier.issn1934-2616de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/6800-
dc.description.abstractLysosomes are terminal degradative organelles that are found in all higher eukaryotic cells. The biogenesis of lysosomes involves the transport of various acid hydrolases and transmembrane glycoproteins from their site of synthesis in the endoplasmic reticulum through the biosynthetic and endocytic pathways. Protein transport to lysosomes can be studied by a combination of techniques based on the separation of intracellular organelles. Percoll density gradient centrifugation has long been the method of choice for separating lysosomes from other organelles in cell homogenates, and accordingly, this unit describes protocols for obtaining reasonably pure lysosomal fractions from mammalian cells using Percoll density gradient separation.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofCurrent Protocols in Cell Biologyde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.titleAnalysis of protein transport to lysosomesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1002/0471143030.cb1508s27de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume27de_CH
zhaw.publication.reviewNot specifiedde_CH
zhaw.webfeedZellphysiologiede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Schaub, B. E., Nair, P., & Rohrer, J. (2005). Analysis of protein transport to lysosomes. Current Protocols in Cell Biology, 27(1). https://doi.org/10.1002/0471143030.cb1508s27
Schaub, B.E., Nair, P. and Rohrer, J. (2005) ‘Analysis of protein transport to lysosomes’, Current Protocols in Cell Biology, 27(1). Available at: https://doi.org/10.1002/0471143030.cb1508s27.
B. E. Schaub, P. Nair, and J. Rohrer, “Analysis of protein transport to lysosomes,” Current Protocols in Cell Biology, vol. 27, no. 1, Jul. 2005, doi: 10.1002/0471143030.cb1508s27.
SCHAUB, Beat E, Prashant NAIR und Jack ROHRER, 2005. Analysis of protein transport to lysosomes. Current Protocols in Cell Biology. 1 Juli 2005. Bd. 27, Nr. 1. DOI 10.1002/0471143030.cb1508s27
Schaub, Beat E, Prashant Nair, and Jack Rohrer. 2005. “Analysis of Protein Transport to Lysosomes.” Current Protocols in Cell Biology 27 (1). https://doi.org/10.1002/0471143030.cb1508s27.
Schaub, Beat E., et al. “Analysis of Protein Transport to Lysosomes.” Current Protocols in Cell Biology, vol. 27, no. 1, July 2005, https://doi.org/10.1002/0471143030.cb1508s27.


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