Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition

Schaub, Beat E.; Berger, Eric G.; Rohrer, Jack

doi:10.1007/s00018-008-8446-y

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-6803

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DC Field	Value	Language
dc.contributor.author	Schaub, Beat E.	-
dc.contributor.author	Berger, Eric G.	-
dc.contributor.author	Rohrer, Jack	-
dc.date.accessioned	2018-06-13T08:33:59Z	-
dc.date.available	2018-06-13T08:33:59Z	-
dc.date.issued	2008-11	-
dc.identifier.issn	1420-9071	de_CH
dc.identifier.issn	1420-682X	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/6803	-
dc.description.abstract	Two major functions of the Golgi apparatus (GA) are formation of complex glycans and sorting of proteins destined for various subcellular compartments or secretion. To fulfill these tasks proper localization of the accessory proteins within the different sub-compartments of the GA is crucial. Here we investigate structural determinants mediating transition of the two glycosyltransferases beta-1,4- galactosyltransferase 1 (gal-T1) and the alpha-1,3-fucosyltransferase 6 (fuc-T6) from the trans-Golgi cisterna to the trans-Golgi network (TGN). Upon treatment with the ionophore monensin both glycosyltransferases are found in TGN-derived swollen vesicles, as determined by confocal fluorescence microscopy and density gradient fractionation. Both enzymes carry a signal consisting of the amino acids E(5)P(6) in gal-T1 and D(2)P(3) in fuc-T6 necessary for the transition of these glycosyltransferases from the trans-Golgi cisterna to the TGN, but not for their steady state localization in the trans-Golgi cisterna.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Springer	de_CH
dc.relation.ispartof	Cellular and Molecular Life Sciences	de_CH
dc.rights	Licence according to publishing contract	de_CH
dc.subject	Golgi Apparatus	de_CH
dc.subject	Sorting signal	de_CH
dc.subject	Trafficking	de_CH
dc.subject	Glycosyltransferases	de_CH
dc.subject	Monensin	de_CH
dc.subject.ddc	571: Physiologie und verwandte Themen	de_CH
dc.title	Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Chemie und Biotechnologie (ICBT)	de_CH
dc.identifier.doi	10.1007/s00018-008-8446-y	de_CH
dc.identifier.doi	10.21256/zhaw-6803	-
zhaw.funding.eu	No	de_CH
zhaw.issue	22	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.pages.end	3687	de_CH
zhaw.pages.start	3677	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	65	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Zellphysiologie	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Schaub, B. E., Berger, E. G., & Rohrer, J. (2008). Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cellular and Molecular Life Sciences, 65(22), 3677–3687. https://doi.org/10.1007/s00018-008-8446-y

Schaub, B.E., Berger, E.G. and Rohrer, J. (2008) ‘Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition’, Cellular and Molecular Life Sciences, 65(22), pp. 3677–3687. Available at: https://doi.org/10.1007/s00018-008-8446-y.

B. E. Schaub, E. G. Berger, and J. Rohrer, “Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition,” Cellular and Molecular Life Sciences, vol. 65, no. 22, pp. 3677–3687, Nov. 2008, doi: 10.1007/s00018-008-8446-y.

SCHAUB, Beat E., Eric G. BERGER und Jack ROHRER, 2008. Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cellular and Molecular Life Sciences. November 2008. Bd. 65, Nr. 22, S. 3677–3687. DOI 10.1007/s00018-008-8446-y

Schaub, Beat E., Eric G. Berger, and Jack Rohrer. 2008. “Dissection of a Novel Molecular Determinant Mediating Golgi to Trans-Golgi Network Transition.” Cellular and Molecular Life Sciences 65 (22): 3677–87. https://doi.org/10.1007/s00018-008-8446-y.

Schaub, Beat E., et al. “Dissection of a Novel Molecular Determinant Mediating Golgi to Trans-Golgi Network Transition.” Cellular and Molecular Life Sciences, vol. 65, no. 22, Nov. 2008, pp. 3677–87, https://doi.org/10.1007/s00018-008-8446-y.