ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes

Crottet, Pascal; Meyer, Daniel M.; Rohrer, Jack; Spiess, Martin

doi:10.1091/mbc.e02-05-0309

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes
Authors:	Crottet, Pascal Meyer, Daniel M. Rohrer, Jack Spiess, Martin
DOI:	10.1091/mbc.e02-05-0309
Published in:	Molecular Biology of the Cell
Volume(Issue):	13
Issue:	10
Page(s):	3672
Pages to:	3682
Issue Date:	Oct-2002
Publisher / Ed. Institution:	American Society for Cell Biology
ISSN:	1939-4586 1059-1524
Language:	English
Subject (DDC):	571: Physiology and related subjects 572: Biochemistry
Abstract:	At the trans-Golgi network, clathrin coats containing AP-1 adaptor complexes are formed in an ARF1-dependent manner, generating vesicles transporting cargo proteins to endosomes. The mechanism of site-specific targeting of AP-1 and the role of cargo are poorly understood. We have developed an in vitro assay to study the recruitment of purified AP-1 adaptors to chemically defined liposomes presenting peptides corresponding to tyrosine-based sorting motifs. AP-1 recruitment was found to be dependent on myristoylated ARF1, GTP or nonhydrolyzable GTP-analogs, tyrosine signals, and small amounts of phosphoinositides, most prominently phosphatidylinositol 4,5-bisphosphate, in the absence of any additional cytosolic or membrane bound proteins. AP-1 from cytosol could be recruited to a tyrosine signal independently of the lipid composition, but the rate of recruitment was increased by phosphatidylinositol 4,5-bisphosphate. The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation.
URI:	https://digitalcollection.zhaw.ch/handle/11475/6813
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Crottet, P., Meyer, D. M., Rohrer, J., & Spiess, M. (2002). ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Molecular Biology of the Cell, 13(10), 3672–3682. https://doi.org/10.1091/mbc.e02-05-0309

Crottet, P. et al. (2002) ‘ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes’, Molecular Biology of the Cell, 13(10), pp. 3672–3682. Available at: https://doi.org/10.1091/mbc.e02-05-0309.

P. Crottet, D. M. Meyer, J. Rohrer, and M. Spiess, “ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes,” Molecular Biology of the Cell, vol. 13, no. 10, pp. 3672–3682, Oct. 2002, doi: 10.1091/mbc.e02-05-0309.

CROTTET, Pascal, Daniel M. MEYER, Jack ROHRER und Martin SPIESS, 2002. ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Molecular Biology of the Cell. Oktober 2002. Bd. 13, Nr. 10, S. 3672–3682. DOI 10.1091/mbc.e02-05-0309

Crottet, Pascal, Daniel M. Meyer, Jack Rohrer, and Martin Spiess. 2002. “ARF1.GTP, Tyrosine-Based Signals, and Phosphatidylinositol 4,5-Bisphosphate Constitute a Minimal Machinery to Recruit the AP-1 Clathrin Adaptor to Membranes.” Molecular Biology of the Cell 13 (10): 3672–82. https://doi.org/10.1091/mbc.e02-05-0309.

Crottet, Pascal, et al. “ARF1.GTP, Tyrosine-Based Signals, and Phosphatidylinositol 4,5-Bisphosphate Constitute a Minimal Machinery to Recruit the AP-1 Clathrin Adaptor to Membranes.” Molecular Biology of the Cell, vol. 13, no. 10, Oct. 2002, pp. 3672–82, https://doi.org/10.1091/mbc.e02-05-0309.