Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology
Authors: Pedan, Vasilisa
Fischer, Norbert
Bernath, Konrad
Hühn, Tilo
Rohn, Sascha
DOI: 10.1016/j.foodchem.2016.07.094
Published in: Food Chemistry
Volume(Issue): 214
Page(s): 523
Pages to: 532
Issue Date: 2017
Publisher / Ed. Institution: Elsevier
ISSN: 0308-8146
Language: English
Subjects: Antioxidant capacity; Chocolate manufacturing process; Oligomeric proanthocyanidins; Antioxidants; Cacao; Chocolate; Polyphenols; Proanthocyanidins
Subject (DDC): 664: Food technology
Abstract: Cocoa beans are a well-known source of antioxidant polyphenols. Especially individual oligomeric proanthocyanidins demonstrated a significant contribution to the total antioxidant activity of cocoa compared to monomeric compounds. An NP-HPLC-online-DPPH assay was developed for separating the homologous series of oligomeric proanthocyanidins and the simultaneous assessment of their antioxidant capacity in relation to the degree of polymerization (DP). The present study describes the influence of the different stages of a lab-scale chocolate manufacturing process on the content of oligomeric proanthocyanidins and their antioxidant capacity. The sum of the total proanthocyanidin content (∑ DP1-DP13) decreased from 30mg epicatechin equivalents per gram non-fat dry matter in raw fresh cocoa beans to 6mg epicatechin equivalents per gram in the final chocolate. The antioxidant capacity decreased accordingly from 25mg epicatechin equivalents per gram non-fat dry matter in raw fresh cocoa beans to 4mg/g in the final chocolate product.
URI: https://digitalcollection.zhaw.ch/handle/11475/7060
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Appears in collections:Publikationen Life Sciences und Facility Management

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Pedan, V., Fischer, N., Bernath, K., Hühn, T., & Rohn, S. (2017). Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology. Food Chemistry, 214, 523–532. https://doi.org/10.1016/j.foodchem.2016.07.094
Pedan, V. et al. (2017) ‘Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology’, Food Chemistry, 214, pp. 523–532. Available at: https://doi.org/10.1016/j.foodchem.2016.07.094.
V. Pedan, N. Fischer, K. Bernath, T. Hühn, and S. Rohn, “Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology,” Food Chemistry, vol. 214, pp. 523–532, 2017, doi: 10.1016/j.foodchem.2016.07.094.
PEDAN, Vasilisa, Norbert FISCHER, Konrad BERNATH, Tilo HÜHN und Sascha ROHN, 2017. Determination of oligomeric proanthocyanidins and their antioxidant capacity from different chocolate manufacturing stages using the NP-HPLC-online-DPPH methodology. Food Chemistry. 2017. Bd. 214, S. 523–532. DOI 10.1016/j.foodchem.2016.07.094
Pedan, Vasilisa, Norbert Fischer, Konrad Bernath, Tilo Hühn, and Sascha Rohn. 2017. “Determination of Oligomeric Proanthocyanidins and Their Antioxidant Capacity from Different Chocolate Manufacturing Stages Using the NP-HPLC-Online-DPPH Methodology.” Food Chemistry 214: 523–32. https://doi.org/10.1016/j.foodchem.2016.07.094.
Pedan, Vasilisa, et al. “Determination of Oligomeric Proanthocyanidins and Their Antioxidant Capacity from Different Chocolate Manufacturing Stages Using the NP-HPLC-Online-DPPH Methodology.” Food Chemistry, vol. 214, 2017, pp. 523–32, https://doi.org/10.1016/j.foodchem.2016.07.094.


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