Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures

Yildirim, Selçuk; Konrad, Daniel; Calvez, Ségolène; Drider, Djamel; Prévost, Hervé; Lacroix, Christophe

doi:10.1007/s00253-007-1188-1

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures
Authors:	Yildirim, Selçuk Konrad, Daniel Calvez, Ségolène Drider, Djamel Prévost, Hervé Lacroix, Christophe
DOI:	10.1007/s00253-007-1188-1
Published in:	Applied Microbiology and Biotechnology
Volume(Issue):	77
Issue:	3
Page(s):	525
Pages to:	531
Issue Date:	Dec-2007
Publisher / Ed. Institution:	Springer
ISSN:	0175-7598 1432-0614
Language:	English
Subject (DDC):	660.6: Biotechnology
Abstract:	To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0-2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06 g cdw/l and 18 ± 1 mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.
URI:	https://digitalcollection.zhaw.ch/handle/11475/7075
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Food and Beverage Innovation (ILGI)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Yildirim, S., Konrad, D., Calvez, S., Drider, D., Prévost, H., & Lacroix, C. (2007). Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures. Applied Microbiology and Biotechnology, 77(3), 525–531. https://doi.org/10.1007/s00253-007-1188-1

Yildirim, S. et al. (2007) ‘Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures’, Applied Microbiology and Biotechnology, 77(3), pp. 525–531. Available at: https://doi.org/10.1007/s00253-007-1188-1.

S. Yildirim, D. Konrad, S. Calvez, D. Drider, H. Prévost, and C. Lacroix, “Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures,” Applied Microbiology and Biotechnology, vol. 77, no. 3, pp. 525–531, Dec. 2007, doi: 10.1007/s00253-007-1188-1.

YILDIRIM, Selçuk, Daniel KONRAD, Ségolène CALVEZ, Djamel DRIDER, Hervé PRÉVOST und Christophe LACROIX, 2007. Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures. Applied Microbiology and Biotechnology. Dezember 2007. Bd. 77, Nr. 3, S. 525–531. DOI 10.1007/s00253-007-1188-1

Yildirim, Selçuk, Daniel Konrad, Ségolène Calvez, Djamel Drider, Hervé Prévost, and Christophe Lacroix. 2007. “Production of Recombinant Bacteriocin Divercin V41 by High Cell Density Escherichia Coli Batch and Fed-Batch Cultures.” Applied Microbiology and Biotechnology 77 (3): 525–31. https://doi.org/10.1007/s00253-007-1188-1.

Yildirim, Selçuk, et al. “Production of Recombinant Bacteriocin Divercin V41 by High Cell Density Escherichia Coli Batch and Fed-Batch Cultures.” Applied Microbiology and Biotechnology, vol. 77, no. 3, Dec. 2007, pp. 525–31, https://doi.org/10.1007/s00253-007-1188-1.