1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Full metadata record
DC FieldValueLanguage
dc.contributor.authorChapleau, Richard R.-
dc.contributor.authorBlomberg, Rebecca-
dc.contributor.authorFord, Peter C.-
dc.contributor.authorSagermann, Martin-
dc.date.accessioned2018-08-27T06:49:17Z-
dc.date.available2018-08-27T06:49:17Z-
dc.date.issued2008-
dc.identifier.issn0961-8368de_CH
dc.identifier.issn1469-896Xde_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/9666-
dc.description.abstractMercury is a ubiquitous pollutant that when absorbed is extremely toxic to a wide variety of biochemical processes. Mercury (II) is a strong, "invisible" poison that is rapidly absorbed by tissues of the intestinal tract, kidneys, and liver upon ingestion. In this study, a novel fluorescence-based biosensor is presented that allows for the direct monitoring of the uptake and distribution of the metal under noninvasive in vivo conditions. With the introduction of a cysteine residue at position 205, located in close proximity to the chromophore, the green fluorescent protein (GFP) from Aequorea victoria was converted into a highly specific biosensor for this metal ion. The mutant protein exhibits a dramatic absorbance and fluorescence change upon mercuration at neutral pH. Absorbance and fluorescence properties with respect to the metal concentration exhibit sigmoidal binding behavior with a detection limit in the low nanomolar range. Time-resolved binding studies indicate rapid subsecond binding of the metal to the protein. The crystal structures obtained of mutant eGFP205C indicate a possible access route of the metal into the core of the protein. To our knowledge, this engineered protein is a first example of a biosensor that allows for noninvasive and real-time imaging of mercury uptake in a living cell. A major advantage is that its expression can be genetically controlled in many organisms to enable unprecedented studies of tissue specific mercury uptake.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofProtein Sciencede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectAbsorptionde_CH
dc.subjectBinding sitesde_CH
dc.subjectBiosensing techniquesde_CH
dc.subjectX-ray crystallographyde_CH
dc.subjectEscherichia colide_CH
dc.subjectGreen Fluorescent Proteinsde_CH
dc.subjectMass spectrometryde_CH
dc.subjectMercuryde_CH
dc.subjectMolecular modelsde_CH
dc.subjectProtein engineeringde_CH
dc.subjectFluorescence spectrometryde_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleDesign of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptakede_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1110/ps.073358908de_CH
dc.identifier.pmid18305194de_CH
zhaw.funding.euNode_CH
zhaw.issue4de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end622de_CH
zhaw.pages.start614de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume17de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedBiokatalysede_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.
Show simple item record
Chapleau, R. R., Blomberg, R., Ford, P. C., & Sagermann, M. (2008). Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake. Protein Science, 17(4), 614–622. https://doi.org/10.1110/ps.073358908
Chapleau, R.R. et al. (2008) ‘Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake’, Protein Science, 17(4), pp. 614–622. Available at: https://doi.org/10.1110/ps.073358908.
R. R. Chapleau, R. Blomberg, P. C. Ford, and M. Sagermann, “Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake,” Protein Science, vol. 17, no. 4, pp. 614–622, 2008, doi: 10.1110/ps.073358908.
CHAPLEAU, Richard R., Rebecca BLOMBERG, Peter C. FORD und Martin SAGERMANN, 2008. Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake. Protein Science. 2008. Bd. 17, Nr. 4, S. 614–622. DOI 10.1110/ps.073358908
Chapleau, Richard R., Rebecca Blomberg, Peter C. Ford, and Martin Sagermann. 2008. “Design of a Highly Specific and Noninvasive Biosensor Suitable for Real-Time in Vivo Imaging of Mercury (II) Uptake.” Protein Science 17 (4): 614–22. https://doi.org/10.1110/ps.073358908.
Chapleau, Richard R., et al. “Design of a Highly Specific and Noninvasive Biosensor Suitable for Real-Time in Vivo Imaging of Mercury (II) Uptake.” Protein Science, vol. 17, no. 4, 2008, pp. 614–22, https://doi.org/10.1110/ps.073358908.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.