Multiparameter microscopy and spectroscopy for single-molecule analytics

Prummer, Michael; Sick, Beate; Renn, Alois; Wild, Urs P.

doi:10.1021/ac034976g

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Multiparameter microscopy and spectroscopy for single-molecule analytics
Authors:	Prummer, Michael Sick, Beate Renn, Alois Wild, Urs P.
DOI:	10.1021/ac034976g
Published in:	Analytical Chemistry
Volume(Issue):	76
Issue:	6
Page(s):	1633
Pages to:	1640
Issue Date:	2004
Publisher / Ed. Institution:	American Chemical Society
ISSN:	0003-2700 1520-6882
Language:	English
Subjects:	Optics; Bayes statistics; Single molecule; Multi parameter
Subject (DDC):	540: Chemistry
Abstract:	The ability to monitor several parameters simultaneously from distinct individual fluorescent reporter molecules facilitates the disentanglement of complex and interacting systems and opens new perspectives in areas from basic science to biopharmaceutical technology. By combining annular illumination microscopy, time-correlated single photon counting, and multichannel detection, we were able to determine 14 independent parameters from one individual fluorophore. The whole set of parameters was deduced from the few properties of the fluorescence photons, i.e., arrival time, wavelength, and polarization. With this approach, the intensity, the polarization, and the spectral dynamics can be analyzed on a nanosecond time scale and the mean values can be monitored with submillisecond time resolution. Nanosecond spectral dynamics of single molecules has been observed, to the best of our knowledge, for the first time. From our experience, we can determine all parameters for more than 30% of the illuminated fluorophores in biological samples and for more than 80% in doped polymeric films.
URI:	https://digitalcollection.zhaw.ch/handle/11475/14032
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	School of Engineering
Organisational Unit:	Institute of Data Analysis and Process Design (IDP)
Appears in collections:	Publikationen School of Engineering

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Prummer, M., Sick, B., Renn, A., & Wild, U. P. (2004). Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry, 76(6), 1633–1640. https://doi.org/10.1021/ac034976g

Prummer, M. et al. (2004) ‘Multiparameter microscopy and spectroscopy for single-molecule analytics’, Analytical Chemistry, 76(6), pp. 1633–1640. Available at: https://doi.org/10.1021/ac034976g.

M. Prummer, B. Sick, A. Renn, and U. P. Wild, “Multiparameter microscopy and spectroscopy for single-molecule analytics,” Analytical Chemistry, vol. 76, no. 6, pp. 1633–1640, 2004, doi: 10.1021/ac034976g.

PRUMMER, Michael, Beate SICK, Alois RENN und Urs P. WILD, 2004. Multiparameter microscopy and spectroscopy for single-molecule analytics. Analytical Chemistry. 2004. Bd. 76, Nr. 6, S. 1633–1640. DOI 10.1021/ac034976g

Prummer, Michael, Beate Sick, Alois Renn, and Urs P. Wild. 2004. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry 76 (6): 1633–40. https://doi.org/10.1021/ac034976g.

Prummer, Michael, et al. “Multiparameter Microscopy and Spectroscopy for Single-Molecule Analytics.” Analytical Chemistry, vol. 76, no. 6, 2004, pp. 1633–40, https://doi.org/10.1021/ac034976g.