Three-dimensional optical polarization tomography of single molecules

Prummer, Michael; Sick, Beate; Hecht, Bert; Wild, Urs P.

doi:10.1063/1.1569848

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Three-dimensional optical polarization tomography of single molecules
Authors:	Prummer, Michael Sick, Beate Hecht, Bert Wild, Urs P.
DOI:	10.1063/1.1569848
Published in:	The Journal of Chemical Physics
Volume(Issue):	118
Issue:	21
Page(s):	9824
Issue Date:	2003
Publisher / Ed. Institution:	American Institute of Physics
ISSN:	0021-9606 1520-9032 1089-7690
Language:	English
Subjects:	Optics; Microscopy; Single molecule; Multi parameter
Subject (DDC):	540: Chemistry
Abstract:	We apply the concept of tomography to polarization-sensitive optical microscopy of single fluorophores to determine the three-dimensional orientation of molecular absorption dipoles with isotropic sensitivity. Wide-field microscopy provides the opportunity to monitor simultaneously three-dimensional rotation and two-dimensional translation of many molecules in parallel. For orientation determination the molecules are illuminated from different directions of incidence with linearly polarized light. In each exposure the excitation along a particular projection of the absorption dipole on the electric field leads to a distinct fluorescence intensity. Five exposures are sufficient to determine the full orientation of the fluorophores. To demonstrate the potential of the method we determine the orientation and position of individual immobilized lipid membrane markers. The shot-noise-limited isotropic angular resolution is 2°. For time-resolved studies the bandwidth can be expanded up to 200 Hz.
URI:	https://digitalcollection.zhaw.ch/handle/11475/14034
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	School of Engineering
Organisational Unit:	Institute of Data Analysis and Process Design (IDP)
Appears in collections:	Publikationen School of Engineering

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Prummer, M., Sick, B., Hecht, B., & Wild, U. P. (2003). Three-dimensional optical polarization tomography of single molecules. The Journal of Chemical Physics, 118(21), 9824. https://doi.org/10.1063/1.1569848

Prummer, M. et al. (2003) ‘Three-dimensional optical polarization tomography of single molecules’, The Journal of Chemical Physics, 118(21), p. 9824. Available at: https://doi.org/10.1063/1.1569848.

M. Prummer, B. Sick, B. Hecht, and U. P. Wild, “Three-dimensional optical polarization tomography of single molecules,” The Journal of Chemical Physics, vol. 118, no. 21, p. 9824, 2003, doi: 10.1063/1.1569848.

PRUMMER, Michael, Beate SICK, Bert HECHT und Urs P. WILD, 2003. Three-dimensional optical polarization tomography of single molecules. The Journal of Chemical Physics. 2003. Bd. 118, Nr. 21, S. 9824. DOI 10.1063/1.1569848

Prummer, Michael, Beate Sick, Bert Hecht, and Urs P. Wild. 2003. “Three-Dimensional Optical Polarization Tomography of Single Molecules.” The Journal of Chemical Physics 118 (21): 9824. https://doi.org/10.1063/1.1569848.

Prummer, Michael, et al. “Three-Dimensional Optical Polarization Tomography of Single Molecules.” The Journal of Chemical Physics, vol. 118, no. 21, 2003, p. 9824, https://doi.org/10.1063/1.1569848.