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  2. School of Engineering
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dc.contributor.authorMiao, Jun-
dc.contributor.authorWu, Wei-
dc.contributor.authorSpielmann, Thomas-
dc.contributor.authorBelfort, Marlene-
dc.contributor.authorDerbyshire, Victoria-
dc.contributor.authorBelfort, Georges-
dc.date.accessioned2019-03-21T13:42:50Z-
dc.date.available2019-03-21T13:42:50Z-
dc.date.issued2005-
dc.identifier.issn1473-0197de_CH
dc.identifier.issn1473-0189de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/16248-
dc.description.abstractSingle-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The linkers were previously derived from self-splicing protein elements called inteins. Two different linkers were generated to solve two distinct separation problems: one for rapid single-step affinity purification of a wide range of proteins, and the other specifically for the purification of cytotoxic proteins. Scale-down factors of 185 resulted in separations in a 27 microl bed-volume. A rotating CD format was chosen because of its simplicity in effecting fluid movement through centrifugal force without the complications associated with electro-osmosis and other pumping methods. The design and fabrication of the fluidic device and the protein purification process are described. This work, which demonstrates the purification of active proteins by two distinct fluidic separations, is widely applicable to small-scale massively parallel proteomic separations.de_CH
dc.language.isoende_CH
dc.publisherRoyal Society of Chemistryde_CH
dc.relation.ispartofLab on a Chipde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectChromatography, Affinityde_CH
dc.subjectEndodeoxyribonucleasesde_CH
dc.subjectEscherichia colide_CH
dc.subjectGene Expression Regulation, Bacterialde_CH
dc.subjectHydrogen-Ion Concentrationde_CH
dc.subjectInteinsde_CH
dc.subjectMicrofluidicsde_CH
dc.subjectProtein Splicingde_CH
dc.subjectProteomicsde_CH
dc.subjectRecombinant Fusion Proteinsde_CH
dc.subjectTime Factorsde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleSingle-step affinity purification of toxic and non-toxic proteins on a fluidics platformde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitute of Materials and Process Engineering (IMPE)de_CH
dc.identifier.doi10.1039/b413292kde_CH
dc.identifier.pmid15726200de_CH
zhaw.funding.euNode_CH
zhaw.issue3de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end253de_CH
zhaw.pages.start248de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume2005de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedVerfahrenstechnikde_CH
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Miao, J., Wu, W., Spielmann, T., Belfort, M., Derbyshire, V., & Belfort, G. (2005). Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab on a Chip, 2005(3), 248–253. https://doi.org/10.1039/b413292k
Miao, J. et al. (2005) ‘Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform’, Lab on a Chip, 2005(3), pp. 248–253. Available at: https://doi.org/10.1039/b413292k.
J. Miao, W. Wu, T. Spielmann, M. Belfort, V. Derbyshire, and G. Belfort, “Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform,” Lab on a Chip, vol. 2005, no. 3, pp. 248–253, 2005, doi: 10.1039/b413292k.
MIAO, Jun, Wei WU, Thomas SPIELMANN, Marlene BELFORT, Victoria DERBYSHIRE und Georges BELFORT, 2005. Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab on a Chip. 2005. Bd. 2005, Nr. 3, S. 248–253. DOI 10.1039/b413292k
Miao, Jun, Wei Wu, Thomas Spielmann, Marlene Belfort, Victoria Derbyshire, and Georges Belfort. 2005. “Single-Step Affinity Purification of Toxic and Non-Toxic Proteins on a Fluidics Platform.” Lab on a Chip 2005 (3): 248–53. https://doi.org/10.1039/b413292k.
Miao, Jun, et al. “Single-Step Affinity Purification of Toxic and Non-Toxic Proteins on a Fluidics Platform.” Lab on a Chip, vol. 2005, no. 3, 2005, pp. 248–53, https://doi.org/10.1039/b413292k.


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