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dc.contributor.authorMathes, Stephanie H.-
dc.contributor.authorWohlwend, Lorenz-
dc.contributor.authorUebersax, Lorenz-
dc.contributor.authorvon Mentlen, Roger-
dc.contributor.authorThoma, Daniel S.-
dc.contributor.authorJung, Ronald E.-
dc.contributor.authorGörlach, Christoph-
dc.contributor.authorGraf-Hausner, Ursula-
dc.date.accessioned2019-03-28T14:40:13Z-
dc.date.available2019-03-28T14:40:13Z-
dc.date.issued2010-
dc.identifier.issn0006-3592de_CH
dc.identifier.issn1097-0290de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/16378-
dc.description.abstractGingival cells of the oral connective tissue are exposed to complex mechanical forces during mastication, speech, tooth movement and orthodontic treatments. Especially during wound healing following surgical procedures, internal and external forces may occur, creating pressure upon the newly formed tissue. This clinical situation has to be considered when developing biomaterials to augment soft tissue in the oral cavity. In order to pre-evaluate a collagen sponge intended to serve as a substitute for autogenous connective tissue grafts (CTGs), a dynamic bioreactor system was developed. Pressure and shear forces can be applied in this bioreactor in addition to a constant medium perfusion to cell-material constructs. Three-dimensional volume changes and stiffness of the matrices were analyzed. In addition, cell responses such as cell vitality and extracellular matrix (ECM) production were investigated. The number of metabolic active cells constantly increased under fully dynamic culture conditions. The sponges remained elastic even after mechanical forces were applied for 14 days. Analysis of collagen type I and fibronectin revealed a statistically significant accumulation of these ECM molecules (P < 0.05-0.001) when compared to static cultures. An increased expression of tenascin-c, indicating tissue remodeling processes, was observed under dynamic conditions only. The results indicate that the tested in vitro cell culture system was able to mimic both the biological and mechanical environments of the clinical situation in a healing wound.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofBiotechnology & Bioengineeringde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectConnective tissuede_CH
dc.subjectHumande_CH
dc.subjectMouth mucosade_CH
dc.subjectOrgan culture techniquede_CH
dc.subjectMechanical stressde_CH
dc.subjectPhysiological stressde_CH
dc.subjectTransplantde_CH
dc.subjectBioreactorde_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc660.6: Biotechnologiede_CH
dc.titleA bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue graftsde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
zhaw.organisationalunitInstitut für Mechanische Systeme (IMES)de_CH
dc.identifier.doi10.1002/bit.22893de_CH
dc.identifier.pmid20683851de_CH
zhaw.funding.euNode_CH
zhaw.issue6de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end1039de_CH
zhaw.pages.start1029de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume107de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
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Mathes, S. H., Wohlwend, L., Uebersax, L., von Mentlen, R., Thoma, D. S., Jung, R. E., Görlach, C., & Graf-Hausner, U. (2010). A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering, 107(6), 1029–1039. https://doi.org/10.1002/bit.22893
Mathes, S.H. et al. (2010) ‘A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts’, Biotechnology & Bioengineering, 107(6), pp. 1029–1039. Available at: https://doi.org/10.1002/bit.22893.
S. H. Mathes et al., “A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts,” Biotechnology & Bioengineering, vol. 107, no. 6, pp. 1029–1039, 2010, doi: 10.1002/bit.22893.
MATHES, Stephanie H., Lorenz WOHLWEND, Lorenz UEBERSAX, Roger VON MENTLEN, Daniel S. THOMA, Ronald E. JUNG, Christoph GÖRLACH und Ursula GRAF-HAUSNER, 2010. A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering. 2010. Bd. 107, Nr. 6, S. 1029–1039. DOI 10.1002/bit.22893
Mathes, Stephanie H., Lorenz Wohlwend, Lorenz Uebersax, Roger von Mentlen, Daniel S. Thoma, Ronald E. Jung, Christoph Görlach, and Ursula Graf-Hausner. 2010. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering 107 (6): 1029–39. https://doi.org/10.1002/bit.22893.
Mathes, Stephanie H., et al. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering, vol. 107, no. 6, 2010, pp. 1029–39, https://doi.org/10.1002/bit.22893.


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