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  2. School of Engineering
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  4. Publikationen School of Engineering
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dc.contributor.authorde Lange, Victoria-
dc.contributor.authorBinkert, Andreas-
dc.contributor.authorVörös, Janos-
dc.contributor.authorBally, Marta-
dc.date.accessioned2017-12-07T10:02:02Z-
dc.date.available2017-12-07T10:02:02Z-
dc.date.issued2011-
dc.identifier.issn1944-8244de_CH
dc.identifier.issn1944-8252de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1732-
dc.description.abstractWe present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 μm spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose, while the larger microparticles are immobilized in the hydrogel matrix. Without major assay optimization, the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can be produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out.de_CH
dc.language.isoende_CH
dc.publisherAmerican Chemical Societyde_CH
dc.relation.ispartofACS Applied Materials & Interfacesde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectHumande_CH
dc.subjectHydrogelde_CH
dc.subjectImmunoglobulin Gde_CH
dc.subjectMicroarray Analysisde_CH
dc.subjectProtein Array Analysisde_CH
dc.subjectTime Factorde_CH
dc.subjectPolyethylene Glycol Dimethacrylatede_CH
dc.subject.ddc540: Chemiede_CH
dc.titleMicroarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray productionde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
dc.identifier.doi10.1021/am100849fde_CH
dc.identifier.pmid21141937de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end57de_CH
zhaw.pages.start50de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume3de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedDiagnostik und Beratungde_CH
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de Lange, V., Binkert, A., Vörös, J., & Bally, M. (2011). Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production. ACS Applied Materials & Interfaces, 3(1), 50–57. https://doi.org/10.1021/am100849f
de Lange, V. et al. (2011) ‘Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production’, ACS Applied Materials & Interfaces, 3(1), pp. 50–57. Available at: https://doi.org/10.1021/am100849f.
V. de Lange, A. Binkert, J. Vörös, and M. Bally, “Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production,” ACS Applied Materials & Interfaces, vol. 3, no. 1, pp. 50–57, 2011, doi: 10.1021/am100849f.
DE LANGE, Victoria, Andreas BINKERT, Janos VÖRÖS und Marta BALLY, 2011. Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production. ACS Applied Materials & Interfaces. 2011. Bd. 3, Nr. 1, S. 50–57. DOI 10.1021/am100849f
de Lange, Victoria, Andreas Binkert, Janos Vörös, and Marta Bally. 2011. “Microarrays Made Easy : Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production.” ACS Applied Materials & Interfaces 3 (1): 50–57. https://doi.org/10.1021/am100849f.
de Lange, Victoria, et al. “Microarrays Made Easy : Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production.” ACS Applied Materials & Interfaces, vol. 3, no. 1, 2011, pp. 50–57, https://doi.org/10.1021/am100849f.


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