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dc.contributor.authorBinkert, Andreas-
dc.contributor.authorStuder, Philipp-
dc.contributor.authorVörös, Janos-
dc.date.accessioned2017-12-07T10:03:56Z-
dc.date.available2017-12-07T10:03:56Z-
dc.date.issued2009-
dc.identifier.issn1613-6810de_CH
dc.identifier.issn1613-6829de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/1733-
dc.description.abstractA novel microwell chip is developed that can be used to detect protein binding in a liquid environment, together with a liquid handling system that allows the performance of assays with picoliter volumes. A PDMS well structure is cast on a planar optical waveguide, providing reaction containers combined with a high-sensitivity fluorescence readout system. Individual wells of the array can be addressed, filled, and rinsed using a contact-mode pin and ring spotter. This allows for immunoassays in a heavily multiplexed way, as all steps of the assay can be individually chosen per well. An array density of over 1000 wells cm(-2) is used for the current experiments. The wells provide a protected liquid environment in which the handling of proteins in their natural state is possible, thus maintaining their activity. The membrane protein annexin V is chosen as a model protein to demonstrate the current possibilities. Annexin V binds to phosphatidylserine (PS) head groups of lipids in a Ca(2+)-dependent manner and is often chosen as a marker for cell apoptosis. Lipid vesicles with and without PS are spotted in individual wells and spontaneously formed a planar lipid bilayer on the bottom of the buffer-filled wells. Annexin V can be used to distinguish between wells containing PS groups previously incorporated in the membrane patches and reference wells without PS head groups. Also, the dependence on the calcium concentration can be shown. Fluorescence readout of the assays is performed using a highly sensitive system based on a planar optical waveguide.de_CH
dc.language.isoende_CH
dc.publisherWileyde_CH
dc.relation.ispartofSmallde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBiological Assayde_CH
dc.subjectBiomimetic Materialde_CH
dc.subjectLipid Bilayerde_CH
dc.subjectMembrane Proteinde_CH
dc.subjectMicrochemistryde_CH
dc.subjectNanostructurede_CH
dc.subjectNanotechnologyde_CH
dc.subjectProtein Array Analysisde_CH
dc.subject.ddc540: Chemiede_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.titleA microwell array platform for picoliter membrane protein assaysde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
dc.identifier.doi10.1002/smll.200801289de_CH
dc.identifier.pmid19242942de_CH
zhaw.funding.euNode_CH
zhaw.issue9de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end1077de_CH
zhaw.pages.start1070de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume5de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedDiagnostik und Beratungde_CH
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Binkert, A., Studer, P., & Vörös, J. (2009). A microwell array platform for picoliter membrane protein assays. Small, 5(9), 1070–1077. https://doi.org/10.1002/smll.200801289
Binkert, A., Studer, P. and Vörös, J. (2009) ‘A microwell array platform for picoliter membrane protein assays’, Small, 5(9), pp. 1070–1077. Available at: https://doi.org/10.1002/smll.200801289.
A. Binkert, P. Studer, and J. Vörös, “A microwell array platform for picoliter membrane protein assays,” Small, vol. 5, no. 9, pp. 1070–1077, 2009, doi: 10.1002/smll.200801289.
BINKERT, Andreas, Philipp STUDER und Janos VÖRÖS, 2009. A microwell array platform for picoliter membrane protein assays. Small. 2009. Bd. 5, Nr. 9, S. 1070–1077. DOI 10.1002/smll.200801289
Binkert, Andreas, Philipp Studer, and Janos Vörös. 2009. “A Microwell Array Platform for Picoliter Membrane Protein Assays.” Small 5 (9): 1070–77. https://doi.org/10.1002/smll.200801289.
Binkert, Andreas, et al. “A Microwell Array Platform for Picoliter Membrane Protein Assays.” Small, vol. 5, no. 9, 2009, pp. 1070–77, https://doi.org/10.1002/smll.200801289.


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