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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes
Authors: Steinmetz, Lukas
Bourquin, Joel
Barosova, Hana
Haeni, Laetitia
Caldwell, Jessica
Milosevic, Ana
Geers, Christoph
Bonmarin, Mathias
Taladriz-Blanco, Patricia
Rothen-Rutishauser, Barbara
Petri-Fink, Alke
et. al: No
DOI: 10.1039/d0nr03330h
Published in: Nanoscale
Issue Date: 13-Aug-2020
Publisher / Ed. Institution: Royal Society of Chemistry
ISSN: 2040-3364
Language: English
Subject (DDC): 620: Engineering
Abstract: Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in thermography to analyse and compare the association trends of epithelial cells, mesothelial cells, and macrophages exposed to gold nanoparticles and multi-walled carbon nanotubes over 24 h. The presence of nanomaterials in the cells was confirmed by dark field and transmission electron microscopy. The results obtained by lock-in thermography for gold nanoparticles were validated with inductively coupled plasma optical emission spectrometry; with data collected showing a good agreement between both techniques. Furthermore, we demonstrate the detection and quantification of carbon nanotube-cell association in a straightforward, non-destructive, and non-intrusive manner without the need to label the carbon nanotubes. Our results display the first approach in utilizing thermography to assess the carbon nanotube amount in cellular environments.
Fulltext version: Published version
License (according to publishing contract): CC BY 3.0: Attribution 3.0 Unported
Departement: School of Engineering
Organisational Unit: Institute of Computational Physics (ICP)
Appears in collections:Publikationen School of Engineering

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