Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-20409
Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes
Authors: Steinmetz, Lukas
Bourquin, Joel
Barosova, Hana
Haeni, Laetitia
Caldwell, Jessica
Milosevic, Ana
Geers, Christoph
Bonmarin, Mathias
Taladriz-Blanco, Patricia
Rothen-Rutishauser, Barbara
Petri-Fink, Alke
et. al: No
DOI: 10.1039/d0nr03330h
10.21256/zhaw-20409
Published in: Nanoscale
Issue Date: 13-Aug-2020
Publisher / Ed. Institution: Royal Society of Chemistry
ISSN: 2040-3364
2040-3372
Language: English
Subject (DDC): 620: Engineering
Abstract: Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in thermography to analyse and compare the association trends of epithelial cells, mesothelial cells, and macrophages exposed to gold nanoparticles and multi-walled carbon nanotubes over 24 h. The presence of nanomaterials in the cells was confirmed by dark field and transmission electron microscopy. The results obtained by lock-in thermography for gold nanoparticles were validated with inductively coupled plasma optical emission spectrometry; with data collected showing a good agreement between both techniques. Furthermore, we demonstrate the detection and quantification of carbon nanotube-cell association in a straightforward, non-destructive, and non-intrusive manner without the need to label the carbon nanotubes. Our results display the first approach in utilizing thermography to assess the carbon nanotube amount in cellular environments.
URI: https://digitalcollection.zhaw.ch/handle/11475/20409
Fulltext version: Published version
License (according to publishing contract): CC BY 3.0: Attribution 3.0 Unported
Departement: School of Engineering
Organisational Unit: Institute of Computational Physics (ICP)
Appears in collections:Publikationen School of Engineering

Files in This Item:
File Description SizeFormat 
2020_Steinmetz_etal_Sensitive-quantification_Nanoscale.pdf3.69 MBAdobe PDFThumbnail
View/Open
Show full item record
Steinmetz, L., Bourquin, J., Barosova, H., Haeni, L., Caldwell, J., Milosevic, A., Geers, C., Bonmarin, M., Taladriz-Blanco, P., Rothen-Rutishauser, B., & Petri-Fink, A. (2020). Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes. Nanoscale. https://doi.org/10.1039/d0nr03330h
Steinmetz, L. et al. (2020) ‘Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes’, Nanoscale [Preprint]. Available at: https://doi.org/10.1039/d0nr03330h.
L. Steinmetz et al., “Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes,” Nanoscale, Aug. 2020, doi: 10.1039/d0nr03330h.
STEINMETZ, Lukas, Joel BOURQUIN, Hana BAROSOVA, Laetitia HAENI, Jessica CALDWELL, Ana MILOSEVIC, Christoph GEERS, Mathias BONMARIN, Patricia TALADRIZ-BLANCO, Barbara ROTHEN-RUTISHAUSER und Alke PETRI-FINK, 2020. Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes. Nanoscale. 13 August 2020. DOI 10.1039/d0nr03330h
Steinmetz, Lukas, Joel Bourquin, Hana Barosova, Laetitia Haeni, Jessica Caldwell, Ana Milosevic, Christoph Geers, et al. 2020. “Rapid and Sensitive Quantification of Cell-Associated Multi-Walled Carbon Nanotubes.” Nanoscale, August. https://doi.org/10.1039/d0nr03330h.
Steinmetz, Lukas, et al. “Rapid and Sensitive Quantification of Cell-Associated Multi-Walled Carbon Nanotubes.” Nanoscale, Aug. 2020, https://doi.org/10.1039/d0nr03330h.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.