Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-20409
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes |
Authors: | Steinmetz, Lukas Bourquin, Joel Barosova, Hana Haeni, Laetitia Caldwell, Jessica Milosevic, Ana Geers, Christoph Bonmarin, Mathias Taladriz-Blanco, Patricia Rothen-Rutishauser, Barbara Petri-Fink, Alke |
et. al: | No |
DOI: | 10.1039/d0nr03330h 10.21256/zhaw-20409 |
Published in: | Nanoscale |
Issue Date: | 13-Aug-2020 |
Publisher / Ed. Institution: | Royal Society of Chemistry |
ISSN: | 2040-3364 2040-3372 |
Language: | English |
Subject (DDC): | 620: Engineering |
Abstract: | Evaluating nanomaterial uptake and association by cells is relevant for in vitro studies related to safe-by-design approaches, nanomedicine or applications in photothermal therapy. However, standard analytical techniques are time-consuming, involve complex sample preparation or include labelling of the investigated sample system with e.g. fluorescent dyes. Here, we explore lock-in thermography to analyse and compare the association trends of epithelial cells, mesothelial cells, and macrophages exposed to gold nanoparticles and multi-walled carbon nanotubes over 24 h. The presence of nanomaterials in the cells was confirmed by dark field and transmission electron microscopy. The results obtained by lock-in thermography for gold nanoparticles were validated with inductively coupled plasma optical emission spectrometry; with data collected showing a good agreement between both techniques. Furthermore, we demonstrate the detection and quantification of carbon nanotube-cell association in a straightforward, non-destructive, and non-intrusive manner without the need to label the carbon nanotubes. Our results display the first approach in utilizing thermography to assess the carbon nanotube amount in cellular environments. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/20409 |
Fulltext version: | Published version |
License (according to publishing contract): | CC BY 3.0: Attribution 3.0 Unported |
Departement: | School of Engineering |
Organisational Unit: | Institute of Computational Physics (ICP) |
Appears in collections: | Publikationen School of Engineering |
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2020_Steinmetz_etal_Sensitive-quantification_Nanoscale.pdf | 3.69 MB | Adobe PDF | View/Open |
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Steinmetz, L., Bourquin, J., Barosova, H., Haeni, L., Caldwell, J., Milosevic, A., Geers, C., Bonmarin, M., Taladriz-Blanco, P., Rothen-Rutishauser, B., & Petri-Fink, A. (2020). Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes. Nanoscale. https://doi.org/10.1039/d0nr03330h
Steinmetz, L. et al. (2020) ‘Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes’, Nanoscale [Preprint]. Available at: https://doi.org/10.1039/d0nr03330h.
L. Steinmetz et al., “Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes,” Nanoscale, Aug. 2020, doi: 10.1039/d0nr03330h.
STEINMETZ, Lukas, Joel BOURQUIN, Hana BAROSOVA, Laetitia HAENI, Jessica CALDWELL, Ana MILOSEVIC, Christoph GEERS, Mathias BONMARIN, Patricia TALADRIZ-BLANCO, Barbara ROTHEN-RUTISHAUSER und Alke PETRI-FINK, 2020. Rapid and sensitive quantification of cell-associated multi-walled carbon nanotubes. Nanoscale. 13 August 2020. DOI 10.1039/d0nr03330h
Steinmetz, Lukas, Joel Bourquin, Hana Barosova, Laetitia Haeni, Jessica Caldwell, Ana Milosevic, Christoph Geers, et al. 2020. “Rapid and Sensitive Quantification of Cell-Associated Multi-Walled Carbon Nanotubes.” Nanoscale, August. https://doi.org/10.1039/d0nr03330h.
Steinmetz, Lukas, et al. “Rapid and Sensitive Quantification of Cell-Associated Multi-Walled Carbon Nanotubes.” Nanoscale, Aug. 2020, https://doi.org/10.1039/d0nr03330h.
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