Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes

Hendrawan, Siufui; Bono, Epifania; Hutter, Albert; Weber, Ursula; Lheman, Jennifer; Baer, Hans U.

doi:10.1002/jbm.b.34738

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes
Authors:	Hendrawan, Siufui Bono, Epifania Hutter, Albert Weber, Ursula Lheman, Jennifer Baer, Hans U.
et. al:	No
DOI:	10.1002/jbm.b.34738
Published in:	Journal of Biomedical Materials Research Part B : Applied Biomaterials
Volume(Issue):	109
Issue:	5
Page(s):	723
Pages to:	732
Issue Date:	16-Oct-2020
Publisher / Ed. Institution:	Wiley
ISSN:	1552-4973 1552-4981
Language:	English
Subjects:	3D PLLA scaffolds; Hepatocyte; Nano-thick collagen coating; Tissue engineering
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	Orthotopic liver transplantation is presently the most effectual method for the treatment of end-stage liver diseases. Though, one major issue is the restricted number of donor organs that are accessible. Hence, liver tissue engineering is under investigation with the goal of restoring liver functions. In this study, we investigated 3D porous scaffolds made of PLLA coated with a nano thick collagen layer (matrices). Primary rat dermal fibroblasts were used in a first study phase to check matrices' cytocompatibility. More than 70% of seeded cells could adhere and remain viable 24 and 48 hours after the seeding. To test the suitability of the matrices for human primary hepatocytes, HepaRG cells were seeded and analyzed for viability, adhesion rate, and functionality such as albumin secretion. About 80% of seeded HepaRG adhered to the scaffolds remaining viable up to 72 hours. Cells were homogeneously distributed in the entire scaffold with albumin secretion increasing with time. Our results indicate that PLLA collagen-coated matrices allow hepatocytes attachment and distribution throughout the 3D structure, as well as support cell functionality. Such matrices have been applied in our clinical phase II trial. Functional hepatocytes were successfully implanted in patients suffering from liver-cirrhosis with higher cell numbers and adhesions rate compared to our previous trial with the first matrix type and a general improvement in clinical condition.
URI:	https://digitalcollection.zhaw.ch/handle/11475/21982
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Hendrawan, S., Bono, E., Hutter, A., Weber, U., Lheman, J., & Baer, H. U. (2020). Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes. Journal of Biomedical Materials Research Part B : Applied Biomaterials, 109(5), 723–732. https://doi.org/10.1002/jbm.b.34738

Hendrawan, S. et al. (2020) ‘Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes’, Journal of Biomedical Materials Research Part B : Applied Biomaterials, 109(5), pp. 723–732. Available at: https://doi.org/10.1002/jbm.b.34738.

S. Hendrawan, E. Bono, A. Hutter, U. Weber, J. Lheman, and H. U. Baer, “Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes,” Journal of Biomedical Materials Research Part B : Applied Biomaterials, vol. 109, no. 5, pp. 723–732, Oct. 2020, doi: 10.1002/jbm.b.34738.

HENDRAWAN, Siufui, Epifania BONO, Albert HUTTER, Ursula WEBER, Jennifer LHEMAN und Hans U. BAER, 2020. Evaluation of 3D PLLA scaffolds coated with nano‐thick collagen as carrier for hepatocytes. Journal of Biomedical Materials Research Part B : Applied Biomaterials. 16 Oktober 2020. Bd. 109, Nr. 5, S. 723–732. DOI 10.1002/jbm.b.34738

Hendrawan, Siufui, Epifania Bono, Albert Hutter, Ursula Weber, Jennifer Lheman, and Hans U. Baer. 2020. “Evaluation of 3D PLLA Scaffolds Coated with Nano‐Thick Collagen as Carrier for Hepatocytes.” Journal of Biomedical Materials Research Part B : Applied Biomaterials 109 (5): 723–32. https://doi.org/10.1002/jbm.b.34738.

Hendrawan, Siufui, et al. “Evaluation of 3D PLLA Scaffolds Coated with Nano‐Thick Collagen as Carrier for Hepatocytes.” Journal of Biomedical Materials Research Part B : Applied Biomaterials, vol. 109, no. 5, Oct. 2020, pp. 723–32, https://doi.org/10.1002/jbm.b.34738.