Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-24196
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses |
Authors: | Alave Reyes-Furrer, Angela De Andrade, Sonia Bachmann, Dominic Jeker, Heidi Steinmann, Martin Accart, Nathalie Dunbar, Andrew Rausch, Martin Bono, Epifania Rimann, Markus Keller, Hansjörg |
et. al: | No |
DOI: | 10.1038/s42003-021-02691-0 10.21256/zhaw-24196 |
Published in: | Communications Biology |
Volume(Issue): | 4 |
Issue: | 1 |
Page(s): | 1183 |
Issue Date: | 14-Oct-2021 |
Publisher / Ed. Institution: | Nature Publishing Group |
ISSN: | 2399-3642 |
Language: | English |
Subjects: | Matrigel; Hydrogel; Bioprinting; Biofabrication; Human skeletal muscle model; Contraction; Alternative method; 3Rs principle; Drug development |
Subject (DDC): | 610.28: Biomedicine, biomedical engineering |
Abstract: | A key to enhance the low translatability of preclinical drug discovery are in vitro human three-dimensional (3D) microphysiological systems (MPS). Here, we show a new method for automated engineering of 3D human skeletal muscle models in microplates and functional compound screening to address the lack of muscle wasting disease medication. To this end, we adapted our recently described 24-well plate 3D bioprinting platform with a printhead cooling system to allow microvalve-based drop-on-demand printing of cell-laden Matrigel containing primary human muscle precursor cells. Mini skeletal muscle models develop within a week exhibiting contractile, striated myofibers aligned between two attachment posts. As an in vitro exercise model, repeated high impact stimulation of contractions for 3 h by a custom-made electrical pulse stimulation (EPS) system for 24-well plates induced interleukin-6 myokine expression and Akt hypertrophy pathway activation. Furthermore, the known muscle stimulators caffeine and Tirasemtiv acutely increase EPS-induced contractile force of the models. This validated new human muscle MPS will benefit development of drugs against muscle wasting diseases. Moreover, our Matrigel 3D bioprinting platform will allow engineering of non-self-organizing complex human 3D MPS. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/24196 |
Fulltext version: | Published version |
License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Published as part of the ZHAW project: | 3D Gewebe TEDD |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Alave Reyes-Furrer, A., De Andrade, S., Bachmann, D., Jeker, H., Steinmann, M., Accart, N., Dunbar, A., Rausch, M., Bono, E., Rimann, M., & Keller, H. (2021). Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses. Communications Biology, 4(1), 1183. https://doi.org/10.1038/s42003-021-02691-0
Alave Reyes-Furrer, A. et al. (2021) ‘Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses’, Communications Biology, 4(1), p. 1183. Available at: https://doi.org/10.1038/s42003-021-02691-0.
A. Alave Reyes-Furrer et al., “Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses,” Communications Biology, vol. 4, no. 1, p. 1183, Oct. 2021, doi: 10.1038/s42003-021-02691-0.
ALAVE REYES-FURRER, Angela, Sonia DE ANDRADE, Dominic BACHMANN, Heidi JEKER, Martin STEINMANN, Nathalie ACCART, Andrew DUNBAR, Martin RAUSCH, Epifania BONO, Markus RIMANN und Hansjörg KELLER, 2021. Matrigel 3D bioprinting of contractile human skeletal muscle models recapitulating exercise and pharmacological responses. Communications Biology. 14 Oktober 2021. Bd. 4, Nr. 1, S. 1183. DOI 10.1038/s42003-021-02691-0
Alave Reyes-Furrer, Angela, Sonia De Andrade, Dominic Bachmann, Heidi Jeker, Martin Steinmann, Nathalie Accart, Andrew Dunbar, et al. 2021. “Matrigel 3D Bioprinting of Contractile Human Skeletal Muscle Models Recapitulating Exercise and Pharmacological Responses.” Communications Biology 4 (1): 1183. https://doi.org/10.1038/s42003-021-02691-0.
Alave Reyes-Furrer, Angela, et al. “Matrigel 3D Bioprinting of Contractile Human Skeletal Muscle Models Recapitulating Exercise and Pharmacological Responses.” Communications Biology, vol. 4, no. 1, Oct. 2021, p. 1183, https://doi.org/10.1038/s42003-021-02691-0.
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