Full metadata record
DC FieldValueLanguage
dc.contributor.authorD'Agostino, Stefania-
dc.contributor.authorRimann, Markus-
dc.contributor.authorGamba, Piergiorgio-
dc.contributor.authorPerilongo, Giorgio-
dc.contributor.authorPozzobon, Michela-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2022-06-18T07:38:27Z-
dc.date.available2022-06-18T07:38:27Z-
dc.date.issued2022-05-30-
dc.identifier.issn2405-8866de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/25128-
dc.descriptionEU COST Action CA16119 CellFitde_CH
dc.description.abstractThe role of the extracellular matrix (ECM) in tumor recurrence and metastasis has been gaining attention. Indeed, not only cellular, but also structural proteins influence migratory and invasive capacity of tumor cells, including growth and resistance to drugs. Therefore, new in vitro tumor models that entail improved ECM formation and deposition are needed. Here, we are developed three-dimensional (3D) models of pediatric soft tissue sarcoma (Rhabdomyosarcoma [RMS]) with the two major subgroups, the embryonal (ERMS) and the alveolar (ARMS) form. We applied macromolecular crowding (MMC) technology to monolayer cultures, spheroids, and 3D bioprinted constructs. In all culture models, exposure to MMC significantly increased ECM deposition. Interestingly, bioprinted constructs showed a collagen and fibronectin matrix architecture that was comparable to that of tumor xenografts. Furthermore, the bioprinted model not only showed tumor cell growth inside the structure but also displayed cell clusters leaving the edges of the bioprinted construct, probably emulating a metastatic mechanism. ARMS and ERMS cells reacted differently in the bioprinted structure. Indeed, the characteristic metastatic behavior was much more pronounced in the more aggressive ARMS subtype. This promising approach opens new avenues for studying RMS microenvironment and creating a platform for cancer drug testing including the native tumor ECM.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofBioprintingde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBioprintingde_CH
dc.subjectRhabdomyosarcoma microenvironmentde_CH
dc.subjectMacromolecular crowdingde_CH
dc.subject3D model of rhabdomyosarcomade_CH
dc.subject.ddc610.28: Biomedizin, Biomedizinische Technikde_CH
dc.titleMacromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma modelde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.bprint.2022.e00213de_CH
zhaw.funding.euNot specifiedde_CH
zhaw.issuee00213de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume27de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeed3D Gewebe und Biofabrikationde_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.