Please use this identifier to cite or link to this item:
https://doi.org/10.21256/zhaw-25128
Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model |
Authors: | D'Agostino, Stefania Rimann, Markus Gamba, Piergiorgio Perilongo, Giorgio Pozzobon, Michela Raghunath, Michael |
et. al: | No |
DOI: | 10.1016/j.bprint.2022.e00213 10.21256/zhaw-25128 |
Published in: | Bioprinting |
Volume(Issue): | 27 |
Issue: | e00213 |
Issue Date: | 30-May-2022 |
Publisher / Ed. Institution: | Elsevier |
ISSN: | 2405-8866 |
Language: | English |
Subjects: | Bioprinting; Rhabdomyosarcoma microenvironment; Macromolecular crowding; 3D model of rhabdomyosarcoma |
Subject (DDC): | 610.28: Biomedicine, biomedical engineering |
Abstract: | The role of the extracellular matrix (ECM) in tumor recurrence and metastasis has been gaining attention. Indeed, not only cellular, but also structural proteins influence migratory and invasive capacity of tumor cells, including growth and resistance to drugs. Therefore, new in vitro tumor models that entail improved ECM formation and deposition are needed. Here, we are developed three-dimensional (3D) models of pediatric soft tissue sarcoma (Rhabdomyosarcoma [RMS]) with the two major subgroups, the embryonal (ERMS) and the alveolar (ARMS) form. We applied macromolecular crowding (MMC) technology to monolayer cultures, spheroids, and 3D bioprinted constructs. In all culture models, exposure to MMC significantly increased ECM deposition. Interestingly, bioprinted constructs showed a collagen and fibronectin matrix architecture that was comparable to that of tumor xenografts. Furthermore, the bioprinted model not only showed tumor cell growth inside the structure but also displayed cell clusters leaving the edges of the bioprinted construct, probably emulating a metastatic mechanism. ARMS and ERMS cells reacted differently in the bioprinted structure. Indeed, the characteristic metastatic behavior was much more pronounced in the more aggressive ARMS subtype. This promising approach opens new avenues for studying RMS microenvironment and creating a platform for cancer drug testing including the native tumor ECM. |
Further description: | EU COST Action CA16119 CellFit |
URI: | https://digitalcollection.zhaw.ch/handle/11475/25128 |
Fulltext version: | Accepted version |
License (according to publishing contract): | CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International |
Restricted until: | 2024-05-26 |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
File | Description | Size | Format | |
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2022_Dagostino-etal_Macromolecular-crowding-extracellular-matrix-deposition-human-rhabdomyosarcoma_AAM.pdf Until 2024-05-26 | Accepted Version | 1.18 MB | Adobe PDF | View/Open |
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D’Agostino, S., Rimann, M., Gamba, P., Perilongo, G., Pozzobon, M., & Raghunath, M. (2022). Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model. Bioprinting, 27(e00213). https://doi.org/10.1016/j.bprint.2022.e00213
D’Agostino, S. et al. (2022) ‘Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model’, Bioprinting, 27(e00213). Available at: https://doi.org/10.1016/j.bprint.2022.e00213.
S. D’Agostino, M. Rimann, P. Gamba, G. Perilongo, M. Pozzobon, and M. Raghunath, “Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model,” Bioprinting, vol. 27, no. e00213, May 2022, doi: 10.1016/j.bprint.2022.e00213.
D’AGOSTINO, Stefania, Markus RIMANN, Piergiorgio GAMBA, Giorgio PERILONGO, Michela POZZOBON und Michael RAGHUNATH, 2022. Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model. Bioprinting. 30 Mai 2022. Bd. 27, Nr. e00213. DOI 10.1016/j.bprint.2022.e00213
D’Agostino, Stefania, Markus Rimann, Piergiorgio Gamba, Giorgio Perilongo, Michela Pozzobon, and Michael Raghunath. 2022. “Macromolecular Crowding Tuned Extracellular Matrix Deposition in a Bioprinted Human Rhabdomyosarcoma Model.” Bioprinting 27 (e00213). https://doi.org/10.1016/j.bprint.2022.e00213.
D’Agostino, Stefania, et al. “Macromolecular Crowding Tuned Extracellular Matrix Deposition in a Bioprinted Human Rhabdomyosarcoma Model.” Bioprinting, vol. 27, no. e00213, May 2022, https://doi.org/10.1016/j.bprint.2022.e00213.
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