Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Development of an ene reductase-based biocatalytic process for the production of flavor compounds
Authors: Papadopoulou, Athena
Peters, Christin
Borchert, Sonja
Steiner, Kerstin
Buller, Rebecca
et. al: No
DOI: 10.1021/acs.oprd.2c00096
Published in: Organic Process Research & Development
Volume(Issue): 26
Issue: 7
Page(s): 2102
Pages to: 2110
Issue Date: 2022
ISSN: 1083-6160
Language: English
Subjects: Old yellow enzyme; Flavor compound; Industrial biocatalysis; Ene reductase; Double-bond reduction
Subject (DDC): 660: Chemical engineering
Abstract: Ene reductases catalyze the biocatalytic reduction of activated alkenes, offering a powerful biobased alternative to metal-catalyzed and organocatalyzed double-bond reductions. With the aim to utilize the natural catalysts for the development of sustainable industrial processes for the flavor and fragrance (F&F) industry, we investigated the synthetic potential of a wild-type ene reductase library consisting of 20 enzymes to produce flavor compounds, including decanal. In our library screening, we identified several ene reductases that could efficiently reduce 2E-decenal as well as other investigated target substrates. Five of the characterized enzymes exhibited high reduction activities even at increased substrate concentrations (10 g/L). By analyzing additional enzyme characteristics (thermostability, solubility, and activity at 10 °C), enzyme Pbr-ER from Pseudomonas brassicacearum was chosen for further characterization and process optimization. Using optimized reaction conditions, the Pbr-ER-catalyzed reduction of 2Edecenal was performed at 100 mL scale at 40 g/L substrate concentration, achieving a high conversion yield (>93%) within 24 h.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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