|Publication type:||Article in scientific journal|
|Type of review:||Peer review (publication)|
|Title:||Development of an ene reductase-based biocatalytic process for the production of flavor compounds|
|Published in:||Organic Process Research & Development|
|Subjects:||Old yellow enzyme; Flavor compound; Industrial biocatalysis; Ene reductase; Double-bond reduction|
|Subject (DDC):||660: Chemical engineering|
|Abstract:||Ene reductases catalyze the biocatalytic reduction of activated alkenes, offering a powerful biobased alternative to metal-catalyzed and organocatalyzed double-bond reductions. With the aim to utilize the natural catalysts for the development of sustainable industrial processes for the flavor and fragrance (F&F) industry, we investigated the synthetic potential of a wild-type ene reductase library consisting of 20 enzymes to produce flavor compounds, including decanal. In our library screening, we identified several ene reductases that could efficiently reduce 2E-decenal as well as other investigated target substrates. Five of the characterized enzymes exhibited high reduction activities even at increased substrate concentrations (10 g/L). By analyzing additional enzyme characteristics (thermostability, solubility, and activity at 10 °C), enzyme Pbr-ER from Pseudomonas brassicacearum was chosen for further characterization and process optimization. Using optimized reaction conditions, the Pbr-ER-catalyzed reduction of 2Edecenal was performed at 100 mL scale at 40 g/L substrate concentration, achieving a high conversion yield (>93%) within 24 h.|
|Fulltext version:||Published version|
|License (according to publishing contract):||Licence according to publishing contract|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Appears in collections:||Publikationen Life Sciences und Facility Management|
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