Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines

Cueni, Claudia; Nytko, Katarzyna J.; Thumser-Henner, Pauline; Weyland, Mathias S.; Rohrer Bley, Carla

doi:10.1002/vms3.266

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25988

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines
Authors:	Cueni, Claudia Nytko, Katarzyna J. Thumser-Henner, Pauline Weyland, Mathias S. Rohrer Bley, Carla
et. al:	No
DOI:	10.1002/vms3.266 10.21256/zhaw-25988
Published in:	Veterinary Medicine and Science
Volume(Issue):	6
Issue:	3
Page(s):	283
Pages to:	289
Issue Date:	2020
Publisher / Ed. Institution:	Wiley
ISSN:	2053-1095 2053-1095
Language:	English
Subjects:	Buprenorphine; Cancer; Dog; Flow cytometry; Opioid receptor; µ-receptor; Animal; Antineoplastic agent; Buprenorphine; Cell line, tumor; Cell proliferation; Doxorubicin; Methadone
Subject (DDC):	630: Agriculture
Abstract:	Opioid receptor activation was shown to enhance the efficacy of anti-neoplastic drugs in several human cancer cell lines. In these cell lines, doxorubicin increased the number of opioid receptors and methadone concurrently enhanced cellular doxorubicin uptake. Triggered through lay press and media, animal owners started to challenge veterinary oncologists with questions about methadone use in anti-cancer therapy. Especially in veterinary medicine, where side effects of chemotherapy are tolerated to a lesser extent and hence smaller doses are given, agents potentiating chemotherapeutic agents would be an optimal approach to treatment. Canine transitional cell carcinoma cells (TCC, K9TCC), canine osteosarcoma cells (OSA, Abrams) and canine hemangiosarcoma cells (HSA, DAL-4) were incubated with different combinations of methadone, buprenorphine and doxorubicin, in order to test inhibition of cell proliferation. Opioid receptor density was assessed with fluorescence-activated cell sorting in drug native and doxorubicin pretreated cells. In TCC and OSA cell lines opioid receptor density increased after doxorubicin pretreatment. In combination treatment, however, we did not find significant potentiation of doxorubicin's inhibitory effect on proliferation in these cell lines. Neither was there a significant increase of the effect of doxorubicin when the opioids were added 24 hr before doxorubicin. Hence, we could not confirm the hypothesis that opioids increase the anti-proliferative effect of the anti-neoplastic drug doxorubicin in any of these canine tumour cell lines. The lack of effect on a cellular level does not warrant a clinical approach to use opioids together with doxorubicin in dogs with cancer.
URI:	https://digitalcollection.zhaw.ch/handle/11475/25988
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	School of Engineering
Organisational Unit:	Institute of Applied Mathematics and Physics (IAMP)
Appears in collections:	Publikationen School of Engineering

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Cueni, C., Nytko, K. J., Thumser-Henner, P., Weyland, M. S., & Rohrer Bley, C. (2020). Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines. Veterinary Medicine and Science, 6(3), 283–289. https://doi.org/10.1002/vms3.266

Cueni, C. et al. (2020) ‘Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines’, Veterinary Medicine and Science, 6(3), pp. 283–289. Available at: https://doi.org/10.1002/vms3.266.

C. Cueni, K. J. Nytko, P. Thumser-Henner, M. S. Weyland, and C. Rohrer Bley, “Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines,” Veterinary Medicine and Science, vol. 6, no. 3, pp. 283–289, 2020, doi: 10.1002/vms3.266.

CUENI, Claudia, Katarzyna J. NYTKO, Pauline THUMSER-HENNER, Mathias S. WEYLAND und Carla ROHRER BLEY, 2020. Methadone does not potentiate the effect of doxorubicin in canine tumour cell lines. Veterinary Medicine and Science. 2020. Bd. 6, Nr. 3, S. 283–289. DOI 10.1002/vms3.266

Cueni, Claudia, Katarzyna J. Nytko, Pauline Thumser-Henner, Mathias S. Weyland, and Carla Rohrer Bley. 2020. “Methadone Does Not Potentiate the Effect of Doxorubicin in Canine Tumour Cell Lines.” Veterinary Medicine and Science 6 (3): 283–89. https://doi.org/10.1002/vms3.266.

Cueni, Claudia, et al. “Methadone Does Not Potentiate the Effect of Doxorubicin in Canine Tumour Cell Lines.” Veterinary Medicine and Science, vol. 6, no. 3, 2020, pp. 283–89, https://doi.org/10.1002/vms3.266.